Pseudomonas plecoglossicida as a novel bacterium for phosphate solubilizing and indole-3-acetic acid-producing from soybean rhizospheric soils of East Java, Indonesia
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Abstract. Astriani M, Zubaidah S, Abadi AL, Suarsini E. 2020. Pseudomonas plecoglossicida as a novel bacterium for phosphate solubilizing and indole-3-acetic acid-producing from soybean rhizospheric soils of East Java, Indonesia. Biodiversitas 21: 578-586. The use of synthetic fertilizers to grow soybean (Glycine max (L.) Merrill) in a long time, can increase the risk of environmental damage. Therefore, the current study aimed to find phosphate solubilizing bacteria that can produce indole-3-acetic acid (IAA) hormone to minimize the use of chemical fertilizers. Superior isolates selected through characterization of phosphate solubilization activity on Pikovskaya medium, screening of IAA producing bacteria, quantitative estimation of phosphate solubilizing activity using a spectrophotometer, hypersensitivity assay, antagonist within isolate assay, and molecular identification of selected bacterial isolates using 16S rRNA sequencing with primer forward 63f and primer reverse 1387r. Among the isolated bacteria, isolate Arj8 showed the highest phosphate solubilizing activity and IAA production. Molecular identification indicated that isolate Arj8 shared 100% similarity with Pseudomonas plecoglossicida. The highest phosphate solubilizing activity (75.39 mg/L) and IAA production (38.89 ppm) recorded on day-3. Multiple potentialities of P. plecoglossicida as phosphate solubilizing and IAA producing bacterium are a novel finding in the development of bioinoculants as bio-fertilizers that can reduce dependency on synthetic chemical fertilizers.
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References
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Tsuno Y, Fujimatsu T, Endo K, Sugiyama, Yazaki K. 2018. Soyasaponins: A new class of root exudates in soybean (Glycine max). Plant cell Physiol 59(2): 366-375.
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Widawati, S. 2011. Phosphate solubilization by bacteria isolated from Laki island coastal ecosystem. BIODIVERSITAS 12(1): 17-21.
Zainuri A, Wardhono A, Sutomo, Ridjal. 2015. Competitiveness improvement strategy of soybean commodity: study of food security in East Java – Indonesia. Agris on-line Papers in Economics and Informatics 3: 99-106.
Apine O, Jadhav JP. 2011. Optimization of medium for indole-3-acetic acid production using Pantoea agglomerans strain PVM. Journal of Applied Microbiology 110: 1235–1244.
Boricha H, Fulekar MH. 2009. Pseudomonas plecoglossicida as a novel organism for the bioremediation of cypermethrin. Biology and Medicine 1(4): 1-10.
Chaiharn M, Lumyong S. 2009. Phosphate solubilization potential and stress tolerance of rhizobacteria from rice soil in Nothern Thailand. World J Microbiol Biotechnol 25: 305-314.
Chanika E, Georgiadou D, Soueref E, Karas P, Karanasios E, Tsiropoulos NG, Tzortzakakis EA, Karpouzas DG. 2010. Isolation of soil bacteria able to hydrolyze both organophosphate and carbamate pesticides. Bioresource Technology, 102: 3184-3192.
Fallo G, Mubarik NR, Triadiati. 2015. Potency of auxin producing and phosphate solubilizing bacteria from dryland in rice paddy field. Research Journal of Microbiology 10(6): 246-259.
Felsenstein. J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4): 783–791.
Fitriyanti D, Mubarik NR, Tjahjoleksono A. 2017. Characterization of phosphate solubilizing bacteria and nitrogen fixing bacteria from limestone mining region. Malaysian Journal of Microbiology 13(3): 147-155.
Gang, S., Sharma, S., Saraf, M., Buck, M., Schumacher, J. 2019. Analysis of Indole-3-Acetic-Acid (IAA) production in Klebsiella by LC-MS/MS and the salkowski method. Bio-Protocol 9(9): 1-9.
Hungria M, Nogueira MA, Araujo RS. 2015. Soybean seed co-inoculation with Bradyrhizobium spp. and Azospirillum brasilense: A New biotechnological tool to improve yield and sustainability. American Journal of Plant Sciences 6: 811-817 .
Jing, H., and Strader, L.C. 2019. Interplay of auxin and cytokinin in lateral root development. International Journal of Molecular Sciences 20(486): 1-12.
Joner EJ, Aarle IM, Vosatka M. 2000. Phosphatase activity of extra-radical arbuscular mycorrhizal hyphae: A review. Plant and Soil 226: 199–210.
Kalayu, G. 2019. Phosphate solubilizing microorganism: promising approach as biofertilizers. International Journal of Agronomy 2019: 1-7.
Kumar K. 2014. Characterization bio-formulation development and shelf-life studies of locally isolated bio-fertilizer strains. Octa Journal of Environmental Research 2(1): 32-37.
Kristanti NE, Rahmawati F, Maksum M. 2017. Analysis of productivity of soybean [Glycinemax (L.) Merr.] for production for farmers in Indonesia. International Conference on Agro-Industry. Bangkok, Thailand, 9-11 November 2016.
Liu J, Tan L, Wang J, Ni H, Li L. 2016. Complete biodegradetion chlorpyrifos by engineered Pseudomonas putida cells expressing surface-immobilized laccases. Chemosphere 157: 200-207.
Li, Z., Zu, C., Wang, C., Yang, J., Yu, H., Wu, H. 2016. Different responses of rhizosphere and non-rhizosphere soil microbial communities to consecutive Piper nigrum L. monoculture. Nature: Scientific Reports, 6: 35825.
Li, M., Guo, R., Yu, F., Chen, X., Zhao, H., Li, H., and Wu, J. 2018. Indole-3-Acetic Acid biosynthesis pathways in the plant beneficial bacterium Arthrobacter pascens ZZ21. International Journal of Molecular Science 19(443): 1-15.
Lynn TM, Win HS, Kyaw EP, Latt ZK, Yu SS. 2013. Characterization of phosphate solubilizing and potassium decomposing strains and study on their effects on tomato cultivation. International Journal of Innovation and Applied Studies 3(4): 959-966.
Mahmoud, W.Y, Antony, I., and Glick, B.R. 2015. Isolation and characterization of endophytic plant growth promoting bacteria from date palm tree (Phoenix dactylifera L.) and their potential role in salinity tolerance. Antonie van Leeuwenhoek 107: 1519-1532.
Majda, M., and Robert, S. 2018. The role of auxin in cell wall expansion. International Journal of Molecular Sciences 19(951): 1-21.
Marchesi JR et al. 1998. Design and evaluation of useful bacterium-spesific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol. 64:795-799.
Mubarik NR, Wibowo RH, Angraini E, Mursyida E, Wahdi E. 2014. Exploration of bacterial diversity at Cirebon quarry [final report]. Quarry Life Award Project
Mulet M, Lalucat J, Valdes EG. 2010. DNA sequence-based analysis of the Pseudomonas species. Environmental Microbiology 12(6): 1513-1530.
Montiel LGH, Contreras CJC, Amador BM, Hermandez LV, Aguilar Q, Contreras. 2017. Efficiency of two inoculation methods of Pseudomonas putida on growth and yield of tomato plants. Journal of Soil Science and Plant Nutrition 17(4): 1003-1012.
Nautiyal CS. 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganism. FEMS Microbiol Lett 170(1): 265-270.
Patten CL, Glick BR. 2002. Role of Pseudomonas putida indole acetic acid in development of the host plant root system. Applied and Environmental Microbiology 68(8): 3795–3801.
Putra AB, Mukaromah A, Wibowo NC. 2017. Analysis of soybean production to achieve soybean self-sufficiency using system dynamic approach. International Joint Conference on Science and Technology, Ayodya Resort Nusa Dua Bali 27-28 September 2017. [Indonesia].
Qin H, and Huang, R. 2018. Auxin controlled by ethylene streers root development. International Journal of Molecular Sciences 19 (3656): 1-13.
Richardson A, Barea JM, Ann MM, Combaret CP. 2009. Acquisition of Phosphorus and Nitrogen in the Rhizosphere and Plant Growth Promotion by Microorganism. Plant Soil 321: 305-339.
Rosas SB, Andres JA, Rovera M, Correa NS. 2006. Phosphate-solubilizing Pseudomonas putida can influence the rhizobia-legume symbio. Soil Biology & Biochemistry 38: 3502-3505.
Sambrook J, Russell DW. 2001. Molecular Cloning a Laboratory Manual. 3rd ed. New York. Cold Spring Harbor Laboratory Pr.
Singh, I. 2018. Plant growth promoting rhizobacteria (PGPR) and their various mechanisms for plant growth enhancement in stressful conditions: a review. European Journal of Biological Research, 8(4): 191-213.
Solomon, C.B., and Bradley, K.W. 2014. Influence of application timings and sublethal rates of synthetic auxin herbicides on soybean. Weed Technology 28: 454-464.
Spaepen S, Vanderleyden J. 2011. Auxin and Plant-Microbe Interactions. Cold Spring Harbor Laboratory Press.
Susila KD, Sudana IM, Ristiati NP, Adnyana, I. M. 2016. Phosphatase activity and phosphate solubilizing rhizobacteria in volcanic soils of Pancasari, Bali. International Journal of Bioscience and Biotechnology 4(1), 39-48.
Tahir M, Mirza MS, Hameed S, Dimitrov MR, Smidt H. 2015. Cultivation-sased and molecular assessment of bacterial diversity in the rhizosheath of Wheat under different crop rotations. Plos One 10(6): 1-28.
Tawaraya K, Horie R, Shinano T, Wagatsuma T, Saito K, Oikawa A. 2014. Metabolite pro?ling of soybean root exudates under phosphorus de?ciency. Soil Science and Plant Nutrition 60: 679-694.
Tsuno Y, Fujimatsu T, Endo K, Sugiyama, Yazaki K. 2018. Soyasaponins: A new class of root exudates in soybean (Glycine max). Plant cell Physiol 59(2): 366-375.
Wahyudi AT, Astuti RP, Widyawati A, Meryandini A. 2011. Characterization of Bacillus sp. strains isolated from rhizosphere of soybean plants for their use as potential plant growth for promoting Rhizobacteria . Journal of Microbiology and Antimicrobials 3(2): 34-40.
Weil, R.R., and Brady, N.C., 2017. Phosphorus and Potassium, Chapter 14, p. 6433-695. The Nature and Properties of Soils, 15th ed. Pearson, Columbus, OH, USA.
Wei, Y., Zhao, Y., Shi, M., Cao, Z., Lu, Q., Yang, T., Fan, Y., Wei, Z., (2017). Effect of organic acids production and bacterial community on the possible mechanism of phosphorus solubilization during composting with enriched phosphate-solubilizing bacteria inoculation, Bioresource Technology, doi: http:// dx.doi.org/10.1016/j.biortech.2017.09.092
Widawati, S. 2011. Phosphate solubilization by bacteria isolated from Laki island coastal ecosystem. BIODIVERSITAS 12(1): 17-21.
Zainuri A, Wardhono A, Sutomo, Ridjal. 2015. Competitiveness improvement strategy of soybean commodity: study of food security in East Java – Indonesia. Agris on-line Papers in Economics and Informatics 3: 99-106.
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