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A Simple Liquid Chromatography Method for the Determination of Captopril in Urine

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Abstract

A simple and specific method for the determination of total captopril in human urine was developed. 2-Chloro-1-methylquinolinium tetrafluoroborate was used as a thiol precolumn derivatizing reagent after conversion of a disulfide forms to free captopril with tris(2-carboxyethyl)phosphine hydrochloride. The 2-S-quinolinium derivative of captopril was separated on a Zorbax SB C-18 column using reversed-phase ion-paring chromatography and monitored by spectrophotometric detector at 355 nm. The calibration curve for the derivatized captopril showed linearity in the range 0.1–200 μmol L−1 of urine with a regression coefficient corresponding to 0.9999. The detection and quantitation limits were 0.05 and 0.1 μmol L−1, respectively. The intra-day imprecision was from 0.01 to 10.58%. This method can be used for routine clinical monitoring of the thiol-drug. Omission of the reduction step gives result for concentration of the reduced form of captopril.

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Acknowledgments

The authors wish to thank the University of Lodz for financial support of this research. K. Kuśmierek is also grateful for the project co-financed by European Union and State Budget of Poland (the GRRI-D Project) for PhD scholarship.

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Authors and Affiliations

  1. Department of Environmental Chemistry, University of Lodz, 163 Pomorska Str, 90-236, Lodz, Poland

    K. Kuśmierek & E. Bald

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  1. K. Kuśmierek
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  2. E. Bald
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Correspondence to E. Bald.

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Kuśmierek, K., Bald, E. A Simple Liquid Chromatography Method for the Determination of Captopril in Urine. Chroma 66, 71–74 (2007). https://doi.org/10.1365/s10337-007-0232-8

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