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HPTLC Method for Determination of 20-Hydroxyecdysone in Sida rhombifolia L. and Dietary Supplements

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Abstract

Naturally occurring 20-hydroxyecdysone is an important anabolic ecdysteroid. A simple thin-layer chromatography method to quantitate 20-hydroxyecdysone in methanolic extract of the whole plant material of Sida rhombifolia L. was developed. This method was successfully applied for quantitative evaluation of dietary supplements. The separation was achieved on glass TLC plates coated with silica gel 60F254, using chloroform: methanol (8:2 v/v) as developing solvent. Densitometric evaluation of 20-hydroxyecdysone was performed at 250 nm in reflectance/absorbance mode. The calibration was in the range of 200–1,000 ng spot−1 and correlation coefficient for the calibration curve was >0.999. In addition, for six different Sida species unique fingerprints were obtained on the HPTLC plate.

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Acknowledgments

This research is funded in part by “Botanical Dietary Supplements: Science-Base for Authentication” funded by Food and Drug Administration grant number FD-U-002071–06. Authors are thankful to Julie Mikell for her suggestions.

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Authors and Affiliations

  1. National Center for Natural Products Research, University of Mississippi, University, MS, 38677, USA

    Atul N. Jadhav, Chidananda Swamy Rumalla, Bharathi Avula & Ikhlas A. Khan

  2. Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University, MS, 38677, USA

    Ikhlas A. Khan

Authors
  1. Atul N. Jadhav
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  2. Chidananda Swamy Rumalla
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  3. Bharathi Avula
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  4. Ikhlas A. Khan
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Correspondence to Ikhlas A. Khan.

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Jadhav, A.N., Rumalla, C.S., Avula, B. et al. HPTLC Method for Determination of 20-Hydroxyecdysone in Sida rhombifolia L. and Dietary Supplements. Chroma 66, 797–800 (2007). https://doi.org/10.1365/s10337-007-0407-3

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  • DOI: https://doi.org/10.1365/s10337-007-0407-3

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