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A Validated and Densitometric HPTLC Method for the Quantification of Withaferin-A and Withanolide-A in Different Plant Parts of Two Morphotypes of Withania somnifera

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Abstract

A simple, sensitive and accurate high performance thin layer chromatographic (HPTLC) method has been developed for the estimation of withaferin-A and withanolide-A in different plant parts such as, leaf, root, stem and fruit of two morphotypes of Withania somnifera. HPTLC of W. somnifera methanolic extract was performed on Si 60 F254 (20 cm × 20 cm) plates with toluene:ethyl acetate:formic acid (5:5:1), as mobile phase. Quantitative evaluation of the plate was performed in the absorption-reflection mode at 530 nm. The method was validated for precision, repeatability, and accuracy. The average recovery of withaferin-A and withanolide-A in two levels were 96.0 and 96.7%, showing the excellent reproducibility of the method. The calibration curves were linear for both in the range of 200–3,200 ng. The technique has been applied, for the first time, for the estimation of withaferin-A and withanolide-A in different parts of the two morphotypes of Withania somnifera. The method is simple, precise, specific, sensitive and accurate and can be used for routine analysis as well as for quality control of raw materials and herbal formulations.

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References

  1. Agarwal R, Diwanay S, Patki P, Patwardhan B (1999) J Ethanopharmocol 67(1):27–35

    Article  CAS  Google Scholar 

  2. Indian Pharmacopoeia (1985) Ashwagandha. In: IP 1985, appendix 3.3:10:69, Ministry of Health and Family welfare, Government of India, Controller of Publications, Delhi

  3. Asthana R, Raina MK (1989) Indian Drugs 26:199–205

    Google Scholar 

  4. Devi PU (1996) Ind J Exp Bio 34:927–932

    CAS  Google Scholar 

  5. Singh S, Kumar S (1998) Central Institute of Med and Arom Plants, p 293

  6. Kirson I, Glotter E, Lavie D, Abraham A (1971) J Chem Soc 2032–2044

  7. Scartezzini P, Speroni E (2000) J Ethnopharmacol 71:23–43

    Article  CAS  Google Scholar 

  8. Agarwal R, Diwanay S, Patwardhan B (1999) J Ethnopharmacol 67:27–35

    Article  CAS  Google Scholar 

  9. Sharada AC, Solomon FE, Devi PU, Udupa N, Srinivasan KK (1996) Acta Oncologica 35:95–100

    CAS  Google Scholar 

  10. Ziauddin M, Pansalkar N, Patki PS, Patwardhan B (1996) J Ethnopharmacol 50:69–76

    Article  CAS  Google Scholar 

  11. Budhiraja RD, Sudhir S (1987) J Sci Ind Res 46:488–491

    CAS  Google Scholar 

  12. Budhiraja RD, Sudhir S (1998) J Sci Ind Res 46:488–491

    Google Scholar 

  13. Ray AB, Gupta M (1994) Prog Chem Org Nat Prod 63:1–106

    CAS  Google Scholar 

  14. Burton G, Veleiro AS, Gros EG (1982) J Chromatog 248:472–475

    Article  CAS  Google Scholar 

  15. Gupta AP, Verma RK, Misra HO, Gupta MM (1996) J Med Arom Plant Sci 18:788–790

    CAS  Google Scholar 

  16. Ganzera M, Choudhary MI, Khan IA (2003) Fitoterapia 74:68–76

    Article  CAS  Google Scholar 

  17. Hunter IR, Walden MK, Heftmann E (1979) J Chromatogr 170:437

    Article  CAS  Google Scholar 

  18. Mahadevan N, Kasar Rahul P, Subburaju T, Suresh B (2003) J Sep Sci 26:1707–1709

    Article  CAS  Google Scholar 

  19. Khajuria RK, Suri KA, Gupta RK, Satti NK, Amina M, Suri OP, Qazi GN, (2004) J Sep Sci 27(7–8):541–546

    Article  CAS  Google Scholar 

  20. Abraham A, Kirson I, Glotter E, Lavie D (1968) J Phytochemistry 7:957

    Article  CAS  Google Scholar 

  21. Kirson I, Glotter E, Lavie D, Abraham A (1971) J Chem Soc C (org) 2032–2044

  22. Chakrabarti SK, De K Barun, Bandopadhyay P (1974) J Experimentia 30:852–853

    Article  Google Scholar 

  23. Bhandari P, Gupta AP, Singh B, Kaul VK (2006) J Planar Chromatography-Modern TLC 19:212–215

    Article  CAS  Google Scholar 

  24. Bhandari P, Gupta AP, Singh B, Kaul VK (2005) J Sep Sci 28:2288–2292

    Article  CAS  Google Scholar 

  25. Mishra N, Gupta AP, Singh B, Kaul VK (2005) J Liq Chromtogr Related Technol 28:665–679

    Google Scholar 

  26. Tripathi AK, Shukla YN, Kumar S, (1996) J Med Arom plant Sci 18:46–62

    Google Scholar 

  27. Sharma K, Dandiya PC (1992) Ind Drugs 29:247

    CAS  Google Scholar 

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Acknowledgments

The authors are greatful to the Director, IHBT, Palampur (H.P) for providing the necessary facilities and this part of work is also supported by SAP-II DRS (UGC), Department of Botany, Punjabi University, Patiala (Punjab).

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Authors and Affiliations

  1. Department of Botany, Punjabi University, Patiala, 147002, Punjab, India

    Vivek Sharma & Raghbir C. Gupta

  2. Natural Plant Products Division, Institute of Himalayan Bioresource Technology, Palampur, 176 061, H.P, India

    Ajai P. Gupta, Pamita Bhandari & Bikram Singh

Authors
  1. Vivek Sharma
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  2. Ajai P. Gupta
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  3. Pamita Bhandari
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  4. Raghbir C. Gupta
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  5. Bikram Singh
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Correspondence to Bikram Singh.

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Sharma, V., Gupta, A.P., Bhandari, P. et al. A Validated and Densitometric HPTLC Method for the Quantification of Withaferin-A and Withanolide-A in Different Plant Parts of Two Morphotypes of Withania somnifera . Chroma 66, 801–804 (2007). https://doi.org/10.1365/s10337-007-0396-2

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