Abstract
This method of RNA extraction relies on a relatively gentle lysis procedure that should burst the cells, but leave the nuclei intact. Contamination of a relatively RNase-free cytoplasmic environment with nuclear nucleases is thus minimised. Next the polysomes are dissociated with SDS and proteinase K and finally the protein is removed by several phenol/choloroform extractions (1,2).
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References
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© 1984 The Humana Press Inc.
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McGookin, R. (1984). RNA Extraction by the Proteinase K Method. In: Walker, J.M. (eds) Nucleic Acids. Methods in Molecular Biology, vol 2. Humana Press. https://doi.org/10.1385/0-89603-064-4:109
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DOI: https://doi.org/10.1385/0-89603-064-4:109
Publisher Name: Humana Press
Print ISBN: 978-0-89603-064-0
Online ISBN: 978-1-59259-489-4
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