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The Bradford Method for Protein Quantitation

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 32))

Abstract

A rapid and accurate method for the estimation of protein concentration is essential in many fields of protein study. An assay originally described by Bradford (1) has become the preferred method for quantifying protein in many laboratories. This technique is simpler, faster, and more sensitive than the Lowry method. Moreover, when compared with the Lowry method, it is subject to less interference by common reagents and nonprotein components of biological samples (see Note 1).

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References

  1. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.

    Article  PubMed  CAS  Google Scholar 

  2. Compton, S. J. and Jones, C. G. (1985) Mechanism of dye response and interference in the Bradford protein assay. Anal. Biochem. 151, 369–374.

    Article  PubMed  CAS  Google Scholar 

  3. Friendenauer, S. and Berlet, H. H. (1989) Sensitivity and variability of the Bradford protein assay in the presence of detergents. Anal. Biochem. 178, 263–268.

    Article  Google Scholar 

  4. Reade, S. M. and Northcote, D. H. (1981) Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein. Anal. Biochem. 116, 53–64.

    Article  Google Scholar 

  5. Stoscheck, C. M. (1990) Increased uniformity in the response of the Coomassie blue protein assay to different proteins. Anal. Biochem. 184, 111–116.

    Article  PubMed  CAS  Google Scholar 

  6. Spector, T. (1978) Refinement of the Coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for <0.5 to 50 µg of protein. Anal. Biochem. 86, 142–146.

    Article  PubMed  CAS  Google Scholar 

  7. Peterson, G. L. (1983) Coomassie blue dye binding protein quantitation method, in Methods in Enzymology, vol. 91 (Hirs, C. H. W. and Timasheff, S. N., eds.), Academic, New York, pp. 95–119.

    Google Scholar 

  8. Wilson, C. M. (1979) Studies and critique of Amido black 10B, Coomassie blue R and Fast green FCF as stains for proteins after polyacrylamide gel electrophoresis. Anal. Biochem. 96, 263–278.

    Article  PubMed  CAS  Google Scholar 

  9. Sedmak, J. J. and Grossberg, S. E. (1977) A rapid, sensitive and versatile assay for protein using Coomassie brilliant blue G250. Anal. Biochem. 79, 544–552.

    Article  PubMed  CAS  Google Scholar 

  10. Redinbaugh, M. G. and Campbell, W. H. (1985) Adaptation of the dye-binding protein assay to microtiter plates. Anal. Biochem. 147, 144–147.

    Article  PubMed  CAS  Google Scholar 

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© 1994 Humana Press Inc., Totowa, NJ

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Kruger, N.J. (1994). The Bradford Method for Protein Quantitation. In: Walker, J.M. (eds) Basic Protein and Peptide Protocols. Methods in Molecular Biology™, vol 32. Humana Press. https://doi.org/10.1385/0-89603-268-X:9

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  • DOI: https://doi.org/10.1385/0-89603-268-X:9

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-268-2

  • Online ISBN: 978-1-59259-519-8

  • eBook Packages: Springer Protocols

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