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Isolation of Genomic DNA from Mycobacteria

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Mycobacteria Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 101))

Abstract

The Mycobacterium genus is comprised of over 30 mdivldual species with a large majority being saphrophytes. However, research on these bacteria has focused primarily on the pathogens Mycobacterium bow, Mycobacterium tuberculosis, and Mycobacterium leprae, and opportunistic pathogens such as the Mycobacterium avium complex and Mycobacterium kansasil. These medically important Mycobacterium spp. have been studied extensively in terms of antigenicity (1,2), pathogenicity (3,4), and physiology (5,6), and with the development of genetic transformation techniques for mycobacteria (7), a new era of dedicated efforts to define these organisms at the genomic level was established Over the past decade, such efforts have been underscored by the isolation of mycobacterial genes encoding immunodommant proteins (2,8–10), virulence factors (11–13) and the biosynthesis of secondary products (14,15); as well as the establishment of genome sequencing projects for M leprae and M. tuberculosis (16,17). Essential to these accomplishments was the development of efficient means to isolate high quality mycobacterial genomic DNA.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Leicester, Leicester, UK

    John T. Belisle

  2. Department of Microbiology, Colorado State University, Fort Collins, CO

    Michael G. Sonnenberg

Authors
  1. John T. Belisle
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  2. Michael G. Sonnenberg
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Editor information

Editors and Affiliations

  1. London School of Hygiene and Tropical Medicine, London, UK

    Tanya Parish  & Neil G. Stoker  & 

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© 1998 Humana Press Inc., Totowa, NJ

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Belisle, J.T., Sonnenberg, M.G. (1998). Isolation of Genomic DNA from Mycobacteria. In: Parish, T., Stoker, N.G. (eds) Mycobacteria Protocols. Methods in Molecular Biology™, vol 101. Humana Press. https://doi.org/10.1385/0-89603-471-2:31

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  • DOI: https://doi.org/10.1385/0-89603-471-2:31

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-471-6

  • Online ISBN: 978-1-59259-576-1

  • eBook Packages: Springer Protocols

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