Abstract
Polyacrylamide gel electrophoresis (PAGE) of whole-cell proteins was first used for differentiation of mycoplasma species by Razin and Rottem (1) The method used was separation through 7.5% acrylamide gels containing 35% acetic acid and 5M urea. However, since the publication of Laemmli’s method for sodium dodecyl sulfate PAGE (SDS-PAGE) (2), most workers have adopted this approach.
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References
Razin, S. and Rottem, S. (1967) Identification of Mycoplasma and other microorganisms by polyacrylamide-gel electrophoresis of cell proteins. J. Bacteriol. 94, 1807–1810.
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Burnette, W. N. (1981) “Western blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulphate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112, 195–203.
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© 1998 Humana Press Inc., Totowa, NJ
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Duffy, M.F., Noormohammadi, A.H., Baseggio, N., Browning, G.F., Markham, P.F. (1998). Polyacrylamide Gel-Electrophoresis Separation of Whole-Cell Proteins. In: Miles, R., Nicholas, R. (eds) Mycoplasma Protocols. Methods in Molecular Biology™, vol 104. Humana Press. https://doi.org/10.1385/0-89603-525-5:267
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DOI: https://doi.org/10.1385/0-89603-525-5:267
Publisher Name: Humana Press
Print ISBN: 978-0-89603-525-6
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