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Restriction Fragment Length Polymorphism Typing of Mycobacteria

  • Protocol
Mycobacterium tuberculosis Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 54))

Abstract

In principle, restriction fragment length polymorphism (RFLP) typing can be applied to strains of all mycobacterial species for which suitable probes have been identified. International consensus has been achieved regarding the methodology of IS6110 RFLP typing of Mycobacterium tuberculosis complex isolates (1) and IS1245 RFLP typing of Mycobacterium avium strains (2). This chapter describes the technical details of these standardized methods regarding the isolation of DNA, restriction enzymes, electrophoresis conditions, internal- and external-size markers, Southern blotting, and several probes used for hybridization. Furthermore, RFLP typing of isolates of some other mycobacterial species is described.

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Authors and Affiliations

  1. Diagnostic Laboratory for Infectious Diseases and Perinatal Screening (Lis), National Institute of Public Health and the Environment (RIVM), Bilhtoven, The Netherlands

    Dick van Soolingen

  2. Diagnostic Laboratory for Infectious Diseases and Perinatal Screening (Lis), National Institute of Public Health and the Environment, Bilhtoven, The Netherlands

    Petra E. W. de Haas & Kristin Kremer

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  1. Dick van Soolingen
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  2. Petra E. W. de Haas
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  3. Kristin Kremer
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Editors and Affiliations

  1. St. Bartholomew’s and Royal London School of Medicine and Dentistry, London, UK

    Tanya Parish

  2. London School of Hygiene and Tropical Medicine, London, UK

    Neil G. Stoker

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van Soolingen, D., de Haas, P.E.W., Kremer, K. (2001). Restriction Fragment Length Polymorphism Typing of Mycobacteria. In: Parish, T., Stoker, N.G. (eds) Mycobacterium tuberculosis Protocols. Methods in Molecular Medicine, vol 54. Humana Press. https://doi.org/10.1385/1-59259-147-7:165

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  • DOI: https://doi.org/10.1385/1-59259-147-7:165

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  • Print ISBN: 978-0-89603-776-2

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