Abstract
Oxygen free radicals (OFR) also known as reactive oxygen species (ROS) are formed as a consequence of normal oxidative metabolism. Their generation goes up substantially under chronic inflammatory conditions and ischemia. These free radicals have multifarious effects that include oxidative damage to DNA leading to various human degenerative diseases. Out of these species, hydroxyl radical is highly reactive and can virtually react with any cell macromolecule, the DNA being the most susceptible. Oxidative damage to DNA includes strand breaks, sister chromatid exchange, and subsequent generation of clastogenetic factors, alterations in basic structure and conformational changes (1 ,2).
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References
Ashok, B. T. and Ali, R. (1999) Mini-review: the aging paradox-free radical theory of aging. Exp. Gerontol. 34, 293–303.
Dizdaroglu, M. (1993) DNA and Free Radicals. Ellis Horwood, Chichester, UK, pp. 19–39.
Stollar, B. D. (1989) Immunochemistry of DNA. Int. Rev. Immunol. 5, 1–22.
Ara, J. and Ali, R. (1993) Polynucleotide specificity of anti-reactive oxygen species (ROS) DNA antibodies. Clin. Exp. Immunol. 94, 134–139.
Arif, Z. and Ali, R. (1996) Antigenicity of poly(dA-dT).poly(dA-dT) photocross-linked with 8-methoxypsoralen. Arch. Biochem. Biophys. 329, 191–198.
Alam, K., Ali, A., and Ali, R. (1993) The effect of hydroxyl radical on the antigenicity of native DNA. FEBS Lett. 319, 66–70.
Pisetsky, D. S. (1996) The immunologic properties of DNA. J. Immunol. 156, 421–23.
Ashok, B. T. and Ali, R. (1998) Binding of circulating antibodies to reactive oxygen species modified-DNA and detecting DNA damage by a monoclonal antibody probe. Mech. Ageing Dev. 103, 69–80.
Hasan, R., Ali, A., and Ali, R. (1991) Antibodies against DNA-psoralen crosslink recognize unique conformation. Biochim. Biophys. Acta 1073, 509–513.
Ali, R., DerSimonian, H., and Stollar, B. D. (1985) Binding of monoclonal anti-native DNA autoantibodies to DNA of varying size and conformation. Mol. Immunol. 22, 1415–1422.
Blakely, W. F., Fuciarelli, A. F., Wegher, B. J., and Dizdaroglu, M. (1990) Hydrogen peroxide-induced base damage in deoxyribonucleic acid. Radiat. Res. 121, 338–343.
Fujiwara, Y., Tatasumi, M., and Sasaki, M.S. (1977) Cross-link repair in human cells and its possible defect in Fanconi’s anemia cells. J. Mol. Biol. 113, 635–649.
Hasan, R. and Ali, R. (1990) Antibody recognition of common epitopes on Z-DNA and native DNA brominated under high salt. Biochem. Int. 20, 1077–1088.
Goding, J. W. (1978) Use of Staphylococcal protein-A as an immunological reagent. J. Immunol. Methods 20, 241–253.
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© 2002 Humana Press Inc.
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Ali, R., Alam, K. (2002). Evaluation of Antibodies Against Oxygen Free Radical-Modified DNA by ELISA. In: Armstrong, D. (eds) Oxidative Stress Biomarkers and Antioxidant Protocols. Methods in Molecular Biology™, vol 186. Humana Press. https://doi.org/10.1385/1-59259-173-6:171
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DOI: https://doi.org/10.1385/1-59259-173-6:171
Publisher Name: Humana Press
Print ISBN: 978-0-89603-850-9
Online ISBN: 978-1-59259-173-2
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