Abstract
Recent developments in PCR fluorimetry have allowed for quick quantification
of target molecules. Before the invention of fluorimetric quantitative PCR, researchers who wanted to quantify the amount of a gene in a sample did so painstakingly by limiting dilution, competitive polymerase chain reaction (PCR), or other methods, including high-performance liquid chromatography (HPLC), solid phase assays, dot blots, or immunoassay (1).
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Wong, A., Cortopassi, G. (2002). Reproducible Quantitative PCR of Mitochondrial and Nuclear DNA Copy Number Using the LightCycler™. In: Copeland, W.C. (eds) Mitochondrial DNA. Methods in Molecular Biology™, vol 197. Humana Press. https://doi.org/10.1385/1-59259-284-8:129
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DOI: https://doi.org/10.1385/1-59259-284-8:129
Publisher Name: Humana Press
Print ISBN: 978-0-89603-972-8
Online ISBN: 978-1-59259-284-5
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