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Hepatitis B and D Protocols pp 125–149Cite as

HBV Genotyping and Analysis for Unique Mutations

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Part of the book series: Methods in Molecular Medicine ((MIMM,volume 95))

Abstract

This chapter describes the methodology, analysis, and assessment of the significance of hepatitis B virus (HBV) mutations selected during antiviral therapy. Included in this description is the methodology for genotype classification. The major area of sequence-analysis methodology focuses on the reverse transcriptase region of the polymerase gene, in particular the catalytic domains A–E. This region coincides with the “a” determinant of the S gene in the overlapping reading frame. Other clinically significant HBV mutations, which are located in other genes and regulatory regions such as the basal core promoter and precore gene [(both of which are associated with loss or reduction in the production of hepatitis Be antigen (HBeAg)], may also be identified. Multiple mutations within a gene and the combination of mutations from other areas of the genome may act in a compensatory manner, altering the resistance pattern, replication phenotype, and pathogenesis profile of viral infection. Thus, the crosslinking of genomic data has potentially important implications for patient management.

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Authors and Affiliations

  1. Victorian Infectious Diseases Reference Laboratory, North Melbourne, Australia

    Anna Ayres, Stephen Locarnini & Angeline Bartholomeusz

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  1. Anna Ayres
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  1. Ribapharm Inc., Costa Mesa, CA

    Robert K. Hamatake PhD  & Johnson Y. N. Lau MD  & 

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Ayres, A., Locarnini, S., Bartholomeusz, A. (2004). HBV Genotyping and Analysis for Unique Mutations. In: Hamatake, R.K., Lau, J.Y.N. (eds) Hepatitis B and D Protocols. Methods in Molecular Medicine, vol 95. Humana Press. https://doi.org/10.1385/1-59259-669-X:125

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  • DOI: https://doi.org/10.1385/1-59259-669-X:125

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-105-9

  • Online ISBN: 978-1-59259-669-0

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