Abstract
Hepadnaviruses utilize an unusual replication strategy. On infection, the partially double-stranded open circular genomic DNA is transported to the hepatocyte nucleus, where host-cell enzymes convert it to a relaxed circular fully double-stranded molecule. From this replicative form is generated a covalently closed circular (ccc) DNA, which associates with cellular histones to form a viral minichromosome (1,2). The HBV (hepatitis B virus) ccc DNA remains in the cell nucleus and serves as the transcriptional template for HBV-RNA production.
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Bowden, S., Jackson, K., Littlejohn, M., Locarnini, S. (2004). Quantification of HBV Covalently Closed Circular DNA from Liver Tissue by Real-Time PCR. In: Hamatake, R.K., Lau, J.Y.N. (eds) Hepatitis B and D Protocols. Methods in Molecular Medicine, vol 95. Humana Press. https://doi.org/10.1385/1-59259-669-X:41
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DOI: https://doi.org/10.1385/1-59259-669-X:41
Publisher Name: Humana Press
Print ISBN: 978-1-58829-105-9
Online ISBN: 978-1-59259-669-0
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