Abstract
Methods to transiently and stably transfect blood stages of the human malaria parasite Plasmodium falciparum have been developed and adapted for gene-knockout, allelic replacement, and transgene expression in this organism. These methods are detailed in this chapter, as are approaches used to monitor transfectants during the selection process. The different plasmid vectors that are currently used for gene targeting and transgene expression (including green fluorescent protein expression) are also described.
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© 2004 Humana Press Inc., Totowa,NJ
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Crabb, B.S. et al. (2004). Transfection of the Human Malaria Parasite Plasmodium falciparum . In: Melville, S.E. (eds) Parasite Genomics Protocols. Methods in Molecular Biology™, vol 270. Humana Press. https://doi.org/10.1385/1-59259-793-9:263
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DOI: https://doi.org/10.1385/1-59259-793-9:263
Publisher Name: Humana Press
Print ISBN: 978-1-58829-062-5
Online ISBN: 978-1-59259-793-2
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