Abstract
The conventional enzyme-linked immunospot (ELISPOT) technique detects only one secreted cytokine, which constitutes a major pitfall for the accurate characterization of the various T-cell subpopulations. We have therefore developed a fluorospot assay, which is a modification of the ELISPOT and is based on the use of multiple fluorescent-labeled anticytokines detection antibodies. A special automated ELISPOT reader consisting of a light microscope with incident fluorescence illumination and an integrating digital color camera has been adapted for this technique. This technique has been applied for the analysis of subpopulations of T-cells and polarized antigen-specific T-cells.
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© 2005 Humana Press Inc., Totowa, NJ
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Gazagne, A., Malkusch, W., Vingert, B., Fridman, W.H., Tartour, E. (2005). Fluorospot Assay. In: Kalyuzhny, A.E. (eds) Handbook of ELISPOT. Methods in Molecular Biology™, vol 302. Humana Press. https://doi.org/10.1385/1-59259-903-6:289
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DOI: https://doi.org/10.1385/1-59259-903-6:289
Publisher Name: Humana Press
Print ISBN: 978-1-58829-469-2
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