Summary
Chromosome analysis is an essential part of the diagnostic testing of myeloid malignancies. Good chromosome preparations are essential for a complete cytogenetic analysis. This means plentiful metaphase spreads with well-spread crisply banded chromosomes. To achieve such a result, several variables, including the growth rate of the leukemic cells, are critical. The method described in this chapter has been extensively tested and should produce reasonable results from most cases. Fluorescence in situ hybridization (FISH) is less influenced by sample variation and as a result may be obtained from either metaphase spreads or interphase cells. Moreover, FISH is capable of describing chromosome rearrangements at the gene level, rather than at the gross level shown by conventional cytogenetics. It does not, however, provide information on genetic rearrangements other than at the specific target site of the probe used, unlike conventional cytogenetics. Thus, these two techniques complement each other and are both now essential elements of chromosome analysis.
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References
Goldman J. M. and Melo J. V. (2003) Chronic myeloid leukemia-advances in biology and new approaches to treatment. N. Engl. J. Med. 349, 1451ā1464.
Byrd J. C., Mrozek K., Dodge R. K., Carroll A. J., Edwards C. G., Arthur D. C., et al. Cancer and Leukemia Group B (CALGB 8461). (2002) Pretreatment cytogenetic abnormalities are predictive of induction success, cumulative incidence of relapse, and overall survival in adult patients with de novo acute myeloid leukemia: results from Cancer and Leukemia Group B (CALGB 8461). Blood 100, 4325ā4336.
Greenberg P., Cox C., LeBeau M. M., Fenaux P., Morel P., Sanz G., et al. (1997) International scoring system for evaluating prognosis in myelodysplastic syndromes. Blood 89, 2079ā2088.
Webber L. M. and Garson O. M. (1983) Fluorodeoxyuridine synchronization of bone marrow cultures. Cancer Genet. Cytogenet. 8, 123ā132.
Barch M. J., Knutsen T., and Spurbeck J. L. (1997) The AGT Cytogenetics Laboratory Manual, third ed. Philadelphia: Lippincott-Raven Publishers.
Frohling S., Skelin S., Liebisch C., Scholl C., Schlenk R. F., Dohner H., et al. Acute Myeloid Leukemia Study Group, Ulm. (2002) Comparison of cytogenetic and molecular cytogenetic detection of chromosome abnormalities in 240 consecutive adult patients with acute myeloid leukemia. J. Clin. Oncol. 20, 2480ā2485.
Sinclair P. B., Nacheva E. P., Leversha M., Telford N., Chang J., Reid A., et al. (2000) Large deletions at the t(9;22) breakpoint are common and may identify a poor-prognosis subgroup of patients with chronic myeloid leukemia. Blood 95, 738ā744.
Andreeff M. and Pinkel D. (1999) Introduction to Fluorescence in situ Hybridization. Wiley-Liss New York.
Mitelman F. (ed) (1995) An International System for Human Cytogenetic Nomenclature. S. Karger, Basel.
Rooney D. E. (2001) Human Cytogenetics: Malignancy and Acquired Abnormalities, third ed. Oxford University Press, Oxford, UK.
Mascarello J. T., Brothman A. R., Davison K., Dewald G. W., Herrman M., McCandless D., et al. Cytogenetics Resource Committee of the College of American Pathologists and American College of Medical Genetics. (2002) Proficiency testing for laboratories performing fluorescence in situ hybridization with chromosome-specific DNA probes. Arch. Pathol. Lab. Med. 126, 1458ā1462.
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Campbell, L.J. (2006). Cytogenetic and FISH Techniques in Myeloid Malignancies. In: Iland, H., Hertzberg, M., Marlton, P. (eds) Myeloid Leukemia. Methods In Molecular Medicineā¢, vol 125. Humana Press. https://doi.org/10.1385/1-59745-017-0:13
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DOI: https://doi.org/10.1385/1-59745-017-0:13
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