Abstract
Several specific problems are encountered when nonradioactive detection methods are used in conjunction with plant nucleic acids. In this chapter, we describe protocols for the isolation of DNA and RNA from plant leaves and the preparation of probe molecules by either PCR or in vitro transcription with different haptens. Furthermore, standard conditions and possible modifications for hybridization and detection of probes are given.
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Peterhaensel, C., Weier, D., Lahaye, T. (2007). Nonradioactive Northern and Southern Analyses From Plant Samples. In: Hilario, E., Mackay, J. (eds) Protocols for Nucleic Acid Analysis by Nonradioactive Probes. Methods in Molecular Biology, vol 353. Humana Press. https://doi.org/10.1385/1-59745-229-7:69
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DOI: https://doi.org/10.1385/1-59745-229-7:69
Publisher Name: Humana Press
Print ISBN: 978-1-58829-430-2
Online ISBN: 978-1-59745-229-8
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