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Invertase production on solid-state fermentation by Aspergillus niger strains improved by parasexual recombination

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Abstract

Invertase production by Aspergillus niger grown by solid-state fermentation was found to be higher than by conventional submerged fermentation. The haploid mutant strains Aw96-3 and Aw96-4 showed better productivity of various enzymes, as compared to wild-type parental strain A. niger C28B25. Here we use parasexual crosses of those mutants to increase further the productivity of invertase in solid-state fermentation. We isolated both a diploid (DAR2) and an autodiploid (AD96-4) strain, which were able to grow in minimal medium after mutation complementation of previously isolated haploid auxotrophic strains. Invertase production was measured in solid-state fermentation cultures, using polyurethane foam as an inert support for fungal growth. Water activity value (Aw) was adjusted to 0.96, since low Aw values are characteristic in some solid-state fermentation processes. Such diploid strains showed invertase productivity levels 5–18 times higher than levels achieved by the corresponding haploid strains. For instance, values for C28B25, Aw96-3, Aw96-4, DAR2, and AD96-4 were 441, 254, 62, 1324, and 2677 IU/(L·h), respectively. These results showed that genetic recombination, achieved through parasexual crosses in A. niger, results in improved strains with potential applications for solid-state fermentation processes.

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Authors and Affiliations

  1. Department of Biotechnology, Universidad Autónoma Metropolitana-Iztapalapa, Apdo. Postal 55-535, C.P. 09340, México D.F., México

    Alba Mónica Montiel-González, Francisco José Fernández, Gustavo Viniegra-González & Octavio Loera

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  1. Alba Mónica Montiel-González
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  2. Francisco José Fernández
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  3. Gustavo Viniegra-González
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  4. Octavio Loera
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Correspondence to Octavio Loera.

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Montiel-González, A.M., Fernández, F.J., Viniegra-González, G. et al. Invertase production on solid-state fermentation by Aspergillus niger strains improved by parasexual recombination. Appl Biochem Biotechnol 102, 63–70 (2002). https://doi.org/10.1385/ABAB:102-103:1-6:063

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