Abstract
The present study characterized the ability of a bacterial cutinase to improve the wettability of raw cotton fabrics by specific hydrolysis of the cutin structure of the cuticle. The effect of cutinase was studied alone and in coreaction with pectin lyase. The changes in both the fabric and the reaction fluid were measured and compared to enzymatic hydrolysis with polygalacturonase, and to chemical hydrolysis with boiling NaOH. Water absorbancy, specific staining, fabric weight loss, and evaporative light-scattering reversephase high-performance liquid chromatography analysis of chloroform extract of the reaction fluid were measured to assess the enzymatic hydrolysis of the cuticle waxy layer. The pattern and extent of hydrolysis of the major cuticle constituents depended on the enzyme type and titers employed and paralleled the degree of wettability obtained. The combination of cutinase and pectin lyase resulted in a synergistic effect. The use of detergents improved enzymatic scouring. The major products released to the reaction medium by the cutinase treatment were identified by gas chromatography/mass spectrometry analysis as C:16 and C:18 saturated fatty acid chains.
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References
Husain, P., Lang, N. K., Henderson, L., Liu, J., and Condon, B. (1999), in Book of Papers, International Conference and Exhibition, American Association of Textile Chemists and Colorists, Charlotte, NC, pp. 170–182.
Hardin, I. R., Li, Y., and Akin, D. (1998), in Enzyme Applications in Fiber Processing, ACS Symposium Series 687, Eriksson, K. E. and Cavaco-Paulo, A., eds., American Chemical Society, Washington, DC, pp. 190–203.
Hartzell, M. M. and Hsieh, Y.-L. (1998), in Enzyme Applications in Fiber Processing, ACS Symposium Series 687, Eriksson, K. E. and Cavaco-Paulo, A., eds., American Chemical Society, Washington, DC, pp. 212–227.
Kolattukudy, P. E. (1980), in The Biochemistry of Plants, vol. 4, Stumpf, P., ed., Academic, New York, pp. 571–642.
Kolattukudy, P. E. (1985), Annu. Rev. Phytopathol. 23, 223–250.
Hartzell, M. M. and Hsieh, Y.-L. (1998), Text. Res. J. 68, 233–241.
Rossner, U. (1993), Melliand Textilber. 74, 144–148.
Buchert, J., Pere, J., Puolakka, A., and Nousiainen, P. (2000), Text. Chem. Color. 32, 48–52.
Li, Y. and Hardin, I. R. (1997), Text. Chem. Color. 29, 71–79.
Li, Y. and Hardin, I. R. (1998), Text. Chem. Color. 30, 23–29.
Cavaco-Paulo, A. (1988), in Enzyme Applications in Fiber Processing, ACS Symposium Series 687, Eriksson, K. E. and Cavaco-Paulo, A., eds., American Chemical Society, Washington, DC, pp. 180–189.
Li, Y. and Hardin, I. R. (1998), Text. Res. J. 68, 671–679.
Traore, M. K. and Buschle-Diller, G. (1999), in Book of Papers, International Conference and Exhibition, American Association of Textile Chemists and Colorists, Charlotte, NC, pp. 183–189.
Sakai, T., Sakamoto, T., Hallaert, J., and Vandamme, E. J. (1993), Adv. App. Microbiol. 39, 213–294.
Krebs Lange, N. (1997), Text. Chem. Color. 29, 23–26.
Csiszar, E., Szakacs, G., and Rusznak, I. (1998), in Enzyme Applications in Fiber Processing, ACS Symposium Series 687, Eriksson, K. E. and Cavaco-Paulo, A., eds., American Chemical Society, Washington, DC, pp. 204–211.
Ueda, M., Koo, H., Wakida, T., and Yoshimura, Y. (1994), Text. Res. J. 64, 615–618.
Purdy, R. E. and Kolattukudy, P. E. (1973), Arch. Biochem. Biophys. 159, 61–69.
Kolattukudy, P. E., Purdy, R. E., and Maiti, I. B. (1981), Methods Enzymol. 71, 652–664.
Spagna, G., Pifferi, P. G., and Martino, A. (1993), J. Chem. Technol. Biotechnol. 57, 379–385.
Dygert, S., Li, L. H., Florida, D., and Thoma, J. A. (1967), Anal. Biochem. 13, 367–374.
American Association of Textile Chemists and Colorists (AATCC). (1977), Technical Manual, vol. 27, American Association of Textile Chemists and Colorists, Research Triangle Park, NC, p. 286.
Krebs Lange, N. (1997), Text. Chem. Color. 29, 23–26.
Hjelmenad, L. M. and Chrambach, A. (1984), Methods Enzymol. 104, 305–319.
Walton, T. J. and Kolattukudy, P. E. (1972), Biochemistry 11, 1885–1896.
Ray, K., Lin, Y. Y., Gerard, H. C., Chen, Z., Osman, S. F., Fett, W. F., Moreau, R. A., and Stark, R. E. (1995), Phytochemistry 38, 1361–1369.
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Degani, O., Gepstein, S. & Dosoretz, C.G. Potential use of cutinase in enzymatic scouring of cotton fiber cuticle. Appl Biochem Biotechnol 102, 277–289 (2002). https://doi.org/10.1385/ABAB:102-103:1-6:277
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DOI: https://doi.org/10.1385/ABAB:102-103:1-6:277