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Culture conditions dictate protease and tannase production in submerged and solid-state cultures of Aspergillus niger Aa-20

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Abstract

Undesirable protease production by Aspergillus niger Aa-20 in submerged culture and solid-state culture was evaluated using different concentrations of tannic acid as sole carbon source in a model system designed for tannase production. Protease production was found to be dependent on the culture system used (submerged culture or solid-state culture) and on the initial tannic acid concentration. Expression of protease activity in submerged culture was higher (up to 10 times) than activity obtained in solid-state culture, using identical culture medium composition. In submerged culture, the lowest final protease activity (0.13 IU) was obtained with the highest tannic acid concentration, while in solid-state culture protease activity was not affected by changes in initial substrate concentration. Absence of detectable proteolytic activity in solid-state culture is related to high production of tannase enzyme. Hence, the use of solid-state culture for fungal enzyme production may allow for higher and more stable enzyme titers present in culture extracts.

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Author notes

  1. Christopher Augur

    Present address: The University of Georgia, Complex Carbohydrate Research Center, 220 Riverbend Road, 30602-4712, Athens, GA, USA

Authors and Affiliations

  1. Food Research Department, Faculty of Chemical Sciences, Universidad Autónoma de Coahuila, Unidad Saltillo, Coah., PO Box 252, 25 000, México

    Cristóbal Noé Aguilar

  2. Biotechnology Department, Universidad Autónoma Metropolitana, Unidad Iztapalapa, PO Box 55-535, 09340, México, D.F.

    Cristóbal Noé Aguilar, Ernesto Favela-Torres & Gustavo Vinegra-González

  3. Institut de Recherche pour le Développement, IRD-France CESB/ESIL, 163 Avenue de Luminy F, 13288, Marseille, Cedex 9, France

    Christopher Augur

Authors
  1. Cristóbal Noé Aguilar
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  2. Ernesto Favela-Torres
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  3. Gustavo Vinegra-González
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  4. Christopher Augur
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Correspondence to Christopher Augur.

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Aguilar, C.N., Favela-Torres, E., Vinegra-González, G. et al. Culture conditions dictate protease and tannase production in submerged and solid-state cultures of Aspergillus niger Aa-20. Appl Biochem Biotechnol 102, 407–414 (2002). https://doi.org/10.1385/ABAB:102-103:1-6:407

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  • DOI: https://doi.org/10.1385/ABAB:102-103:1-6:407

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