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Simultaneous saccharification and cofermentation of dilute-acid pretreated yellow poplar hardwood to ethanol using xylose-fermenting Zymomonas mobilis

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Abstract

Simultaneous saccharification and cofermentation (SSCF) was carried out at approximately 15% total solids using conditioned dilute-acid pretreated yellow poplar feedstock, an adapted variant of National Renewable Energy Laboratory (NREL) xylose-fermenting Zymomonas mobilis and either commercial or NREL-produced cellulase enzyme preparations. In 7 d, at a cellulase loading of 12 filter paper units pergram cellulose (FPU/g), the integrated system produced more than 3% w/v ethanol and achieved 54% conversion of all potentially available biomass sugars (total sugars) entering SSCF. A control SSCF employing Sigmacell cellulose and a commercial cellulase at an enzyme loading of 14 FPU/gachieved 65% conversion of total sugars to ethanol.

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Authors and Affiliations

  1. Biotechnology Center for Fuels and Chemicals, National Renewable Energy Laboratory, 1617 Cole Boulevard, 80401-3393, Golden, CO

    James D. McMillan, Mildred M. Newman, David W. Templeton & Ali Mohagheghi

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  1. James D. McMillan
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  2. Mildred M. Newman
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  3. David W. Templeton
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  4. Ali Mohagheghi
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Correspondence to James D. McMillan.

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McMillan, J.D., Newman, M.M., Templeton, D.W. et al. Simultaneous saccharification and cofermentation of dilute-acid pretreated yellow poplar hardwood to ethanol using xylose-fermenting Zymomonas mobilis . Appl Biochem Biotechnol 79, 649–665 (1999). https://doi.org/10.1385/ABAB:79:1-3:649

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