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Enhanced formation of extracellular laccase activity by the white-rot fungus Trametes multicolor

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Abstract

The white-rot fungus Trametes multicolor MB 49 has been identified as an excellent producer of the industrially important enzyme laccase. The formation of extracellular laccase could be considerably stimulated by the addition of Cu(II) to a simple, glycerol-based culture medium. In this study, optimal concentrations of copper were found to be 0.5–1 mM, which were added during the growth phase of the fungus. Other medium components important for laccase production are the carbon and nitrogen sources employed. When using an optimized medium containing glycerol (40 g/L), peptone from meat (15 g/L), and MgSO4·7H2O and stimulating enzyme formation by the addition of 1.0 mM Cu, maximal laccase activities obtained in shake-flask cultures were approx 85 U/mL. These results, however, could not be scaled up to a laboratory fermentor cultivation. Laccase production by T. multicolor decreased considerably when the fungus was grown in a stirred-tank reactor, presumably because of damage of the mycelia caused by shear stress and/or changes in the morphology of the fungus.

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Authors and Affiliations

  1. Division of Biochemical Engineering, Institute of Food Technology, Austria

    Johann Hess, Christian Leitner, Christiane Galhaup, Klaus D. Kulbe & Dietmar Haltrich

  2. Institute of Chemistry, University of Agricultural Sciences Vienna (Universität für Bodenkultur Wien), Muthgasse 18, A-1190, Wien, Austria

    Barbara Hinterstoisser

  3. Fritz Egger Ges.m.b.H, A-3105, St. Pölten-Unterradlberg, Austria

    Martin Steinwender

  4. Wood Composite and Chemistry Center, Austria (Wood Kplus), Area 1, Muthgasse 18, A-1190, Wien, Austria

    Christiane Galhaup, Barbara Hinterstoisser, Martin Steinwender & Dietmar Haltrich

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  1. Johann Hess
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  2. Christian Leitner
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  3. Christiane Galhaup
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  7. Dietmar Haltrich
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Correspondence to Dietmar Haltrich.

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Hess, J., Leitner, C., Galhaup, C. et al. Enhanced formation of extracellular laccase activity by the white-rot fungus Trametes multicolor . Appl Biochem Biotechnol 98, 229–241 (2002). https://doi.org/10.1385/ABAB:98-100:1-9:229

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