Abstract
Studies show mixed conclusions about acute responses of copper status to strenuous exercise. Because copper function involves metalloenzyme activities, which might take days to change, the present study examined the response of three copper metalloenzyme activities to sustained strenuous exercise in sled dogs. A race lasting 12–15 d depressed activities for both plasma ceruloplasmin and erythrocyte superoxide dismutase in dogs consuming commercial dog foods and meats. A shorter, 3-d training run for dogs fed a commercial balanced diet also depressed ceruloplasmin activities but not superoxide dismutase activities. Dogs fed the same diet but that did not run showed no changes in either parameter. Activities of a third copper enzyme, plasma diamine oxidase, also decreased after a 3-d training run. In summary, blood activities of three copper enzymes were depressed by sustained strenuous exercise in sled dogs.
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H. C. Lukaski, Micronutrients (magnesium, zinc, and copper): are mineral supplements needed for athletes?, Int. J. Sport Nutr. 5, S74-S83 (1995).
J. M. C. Howell and J. M. Gawthorne, Copper in Animals and Man, CRC, Boca Raton, FL (1987).
I. Fridovich, Superoxide radical and superoxide dismutases, Annu. Rev. Biochem. 64, 97–112 (1995).
A. Hasegawa, S. Suzuki, Y. Matsumotot, et al., In vivo fatiguing contraction of rat diaphragm produces hydroxyl radicals, Free Radical Biol. Med. 22, 349–354 (1997).
M. Kanter, Free radicals and exercise: effects of nutritional antioxidant supplementation, Exerc. Sports Sci. Rev. 23, 375–397 (1995).
J. Karlsson, Antioxidants and Exercise, Human Kinetics, Champaign, IL (1997).
R. A. Anderson, N. A. Bryden, M. M. Polansky, et al., Acute exercise effects on urinary losses and serum concentrations of copper and zinc of moderately trained and untrained men consuming a controlled diet, Analyst 120, 867–870 (1995).
O. I. Aruoma, T. Reilly, D. MacLaren, et al., Iron, copper and zinc concentrations in human sweat and plasma; the effect of exercise, Clin. Chim. Acta 177, 81–87 (1988).
D. Bordin, L. Sartorelli, G. Bonanni, et al., High intensity physical exercise induced effects on plasma levels of copper and zinc, Biol. Trace Element Res. 36, 129–134 (1993).
W. W. Campbell and R. A. Anderson, Effects of aerobic exercise and training on the trace minerals chromium, zinc and copper, Sports Med. 4, 9–18 (1987).
I. Cordova, M. Gimenez, and J. F. Escanero, Effect of swimming to exhaustion, at low temperatures, on serum Zn, Cu, Mg and Ca in rats, Physiol. Behav. 48, 595–598 (1990).
P. A. Deuster, S. B. Kyle, A. Singh, et al., Exercise-induced changes in blood minerals, associated proteins and hormones in women athletes, J. Sports Med. Phys. Fitness 31, 552–560 (1991).
M. Marrella, F. Guerrini, P. L. Solero, et al., Blood copper and zinc changes in runners after a marathon, J. Trace Elements Electrolytes Health Dis. 7, 248–250 (1993).
R. A. DiSilvestro, A. A. Jones, D. Smith, et al., Plasma diamine oxidase activities in renal dialysis patients, a human with spontaneous copper deficiency and marginally copper deficient rats, Clin. Biochem. 30, 559–563 (1997).
R. A. DiSilvestro and J. T. Marten, Effects of inflammation and copper intake on rat liver and erythrocyte Cu−Zn superoxide dismutase activity levels, J. Nutr. 120, 1223–1227 (1990).
A. A. Jones, R. A. DiSilvestro, M. Coleman, et al., Copper supplementation of adult men: effects on blood copper enzyme activities & indicators of cardiovascular disease risk, Metabolism 46, 1380–1383 (1997).
E. Rice, Standardization of ceruloplasmin activity in terms of International Enzyme Units, Anal. Biochem. 3, 452–456 (1962).
R. A. DiSilvestro, T. L. Foltz, J. Davidson, et al., Effects of copper intake and exercise training on copper antioxidant enzyme activities in rats, J. Trace Elements Exp. Med. 5, 221–225 (1992).
M. B. Harris and J. W. Starnes, Effects of body temperature during exercise training on myocardial adaptations, Am. J. Physiol. (Heart. Circ. Physiol.) 280, H2271-H2280 (2001).
H. C. Lukaski, Effects of exercise training on human copper and zinc nutriture, Adv. Exp. Med. Biol. 258, 163–170 (1989).
S. Oh-ishi, T. Kizaki, J. Nagasawa, et al., Effects of endurance training on superoxide dismutase activity, content and mRNA expression in rat muscle, Clin. Exp. Pharmacol. Physiol. 24, 326–332 (1997).
I. Pereira, L. F. Rosa, D. A., Safi, et al., Antioxidant enzyme activities in the lymphoid organs and muscles of rats fed fatty acids-rich diets subjected to prolonged physical exercise-training, Physiol. Behav. 56, 1049–1055 (1994).
A. Resina, S. Fedi, L. Gatteschi, et al., Comparison of some serum copper parameters in trained runners and control subjects, Int. J. Sports Med. 11, 58–60 (1990).
Resina, L. Gatteschi, M. G. Rubenni, et al., Comparison of some serum copper parameters in trained professional soccer players and control subjects, J. Sports Med. Phys. Fitness 31, 413–416 (1991).
J. Prohaska, Changes in tissue growth, concentrations of copper, iron cytochrome oxidase and superoxide dismutase subsequent to dietary or genetic copper deficiency in mice, J. Nutr. 113, 2048–2058 (1983).
K. Takagi, M. Nakao, and Y. Ogura, Sensitive colorimetric assay of serum diamine oxidase, Clin. Chim. Acta 226, 67–75 (1994).
W. A. Widicus and J. R. Kirk, High pressure liquid chromatographic determination of vitamins A and E in cereal products, J. Assoc. Offic. Anal. Chem. 62, 637–641 (1979).
T. L. Wagner, R. A. DiSilvestro, and D. M. Medeiros, Marginal copper intake and inflammation effects on copper enzyme activities in rats, J. Trace Elements Exp. Med. 8, 41–46 (1995).
A. I. Moldoveanu, R. J. Shephard, and P. N. Shek, The cytokine response to physical activity and training, Sports Med 31, 115–144 (2001).
R. A. DiSilvestro and R. J. Cousins, Glucocorticoid independent mediation of interleukin-1 induced changes in serum zinc and liver metallothionein, Life Sci. 35, 2113–2118 (1984).
R. A. DiSilvestro and R. J. Cousins, Mediation of endotoxin induced changes in zinc metabolism, Am. J. Physiol. 47, E436-E441 (1984).
R. J. Cousins, Absorption, transport, and hepatic metabolism of copper and zinc: special reference to metallothionein and ceruloplasmin, Physiol. Rev. 65, 238–308 (1985).
D. G. Rowbottom and K. J. Green, Acute exercise effects on the immune system, Med. Sci. Sports Exerc. 32, S396-S405 (2001).
R. A. DiSilvestro, Effects of copper intake, inflammation and copper injection on rat serum ceruloplasmin activity levels, Nutr. Res. 10, 355–358 (1990).
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DiSilvestro, R.A., Hinchcliff, K.W. & Blostein-Fujii, A. Sustained strenuous exercise in sled dogs depresses three blood copper enzyme activities. Biol Trace Elem Res 105, 87–96 (2005). https://doi.org/10.1385/BTER:105:1-3:087
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DOI: https://doi.org/10.1385/BTER:105:1-3:087