Abstract
Lead is one of the most hazardous environmental toxins known. The assessment of lead in dental hard tissues is important in the understanding of its to xic effects on oral tissues and in estimating exposure and body burden in individuals exposed to lead from the environment. However, current information on the uptake and distribution of lead in enamel and dentine is limited. The aim of this project was to study, at high resolution, the spatial distribution of lead in enamel and coronal dentine using an experimental rat model. A dose of 40 mg/L of lead nitrate was administered to pregnant femake rats during the periods of gestation and lactation through drinking water. First mandibular molar teeth were removed from their 15-d-old pups and the distribution of lead was studied using a nuclear microprobe (NMP). The distribution of lead in enamel and coronal dentine showed four distinct zones with significantly different mean lead concentrations (p<0.05). High levels of lead were observed in the superficial regions of enamel and in the dentine directly adjacent to the pulp. Additionally, the results confirmed that the NMP is capable of mapping the distribution of lead in teeth at micron resolutions with a detection limit of approx 1 μg/g.
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References
H. L. Needleman and D. Bellinger, The health effects of low level exposure to lead, Annu. Rev. Public Health 12, 111–140 (1991).
L. Jarup, Hazards of heavy metal contamination, Br. Med. Bull. 68, 167–182 (2003).
J. G. Pounds, G. J. Long, and J. F. Rosen, Cellular and molecular toxicity of lead in bone, Environ. Health Perspect. 91, 17–32 (1991).
W. H. Bowen, Exposure to metal ions and susceptibility to dental caries, J. Dent. Educ. 65, 1046–1053 (2001).
G. E. Watson, B. A. Davis, R. F. Raubertas, S. K. Pearson, and W. H. Bowen, Influence of maternal lead ingestion on caries in rat pups, Nat. Med. 3, 1024–1025 (1997).
H. L. Needleman, C. Gunnoe, A. Leviton, et al., Deficits in psychologic and classroom performance of children with elevated dentine lead levels, N. Engl. J. Med. 300, 689–695 (1979).
M. B. Rabinowitz, J. D. Wang, and W. T. Soong, Dentine lead and child intelligence in Taiwan, Arch. Environ. Health 46, 351–360 (1991).
G. Winneke, L. Altmann, U. Kramer, et al., Neurobehavioral and neurophysiological observations in six year old children with low lead levels in East and West Germany, Neurotoxicology 15, 705–713 (1994).
M. Smith, T. Delves, R. Lansdown, B. Clayton, and P. Graham, The effects of lead exposure on urban children: the Institute of Child Health/Southampton Study, Dev. Med. Child. Neurol. 47(Suppl.), 1–54 (1983).
J. Begerow, I. Freier, M. Turfeld, U. Kramer, and L. Dunemann, Internal lead and cadmium exposure in 6-year-old children from western and eastern Germany, Int. Arch. Occup. Environ. Health 66, 243–248 (1994).
S. R. Grobler, R. J. Rossouw, and D. Kotze, Lead in teeth of weanling rats received via the maternal drinking water, Arch. Oral. Biol. 30, 509–511 (1985).
S. R. Grobler, R. J. Rossouw, T. J. v. W. Kotze, and I. A. Stander, The effect of airborne lead on lead levels of blood, incisors and alveolar bone of rats, Arch. Oral Biol. 36, 357–360 (1991).
M. Kaplan, H. J. Persie, and M. Jeffcoat, Lead content of blood and deciduous teeth in lead-exposed Beagle pups, in Low Level Lead Exposure, H. L. Needleman, ed., Raven, New York, pp. 221–230 (1980).
R. D. Evans, P. Richner, and P. M. Outridge, Micro-spatial variations of heavy metals in the teeth of walrus as determined by laser ablation ICP-MS: the potential for reconstructing a history of metal exposure, Arch. Environ. Contam. Toxicol. 28, 55–60 (1995).
P. Budd, J. Montgomery, A. Cox, P. Krause, B. Barreiro, and R. G. Thomas, The distribution of lead within ancient and modern human teeth: implications for long-term and historical exposure monitoring, Sci. Total Environ. 220, 121–136 (1998).
J. E. Ericson, Enamel lead biomarker for prenatal exposure assessment, Environ. Res. 87, 136–140 (2001).
M. M. Hoffman and I. Schour, Quantitative studies in the development of the rat molar. I. The growth pattern of the primary and secondary dentin (from birth to 500 days of age), Anat. Res. 78, 233–251 (1940).
B. Momcilovic, Lead metabolism in lactation, Experimentia 35, 517–518 (1979).
C. G. Ryan, D. N. Jamieson, W. L. Griffin, G. Cripps, and R. Szymanski, The new CSIRO-GEMOC nuclear microprobe: first results, performance and recent applications, Nucl. Instrum. Methods B 181, 12–19 (2001).
C. G. Ryan, Developments in dynamic analysis for quantitative PIXE true elemental imaging, Nucl. Instrum. Methods B 181, 170–179 (2001).
S. R. Malik and J. H. Fremlin, A study of lead distribution in human teeth using charged particle activation analysis, Caries Res. 8, 283–292 (1974).
M. A. Crenshaw and Y. Takano, Mechanisms by which the enamel organ controls calcium entry into the developing enamel, J. Dent. Res. 61(Special Issue), 1574–1579 (1982).
T. Aoba and E. C. Moreno, Changes in the nature and composition of enamel mineral during porcine amelogenesis, Calcif. Tissue Int. 47, 356–364 (1990).
N. G. Purchase and J. E. Fergusson, Lead in teeth: the influence of the tooth type and the sample within a tooth on lead levels, Sci. Total Environ. 52, 239–250 (1986).
A. R. Ten Cate, Oral Histology: Development, Structure and Function, 5th ed., Mosby, St. Louis, MO, pp. 151 (1998).
P. Herr, J. Holz, and L. J. Baume, Mantle dentine in man—a quantitative microradiographic study, J. Biol. Bucc. 14, 139–146 (1986).
U. Stratmann, K. Schaarschmidt, H. P. Wiesmann, U. Plate, H. J. Hohling, and T. Szuwart, The mineralisation of mantle dentine and of circumpulpal dentine in the rat: an ultrastructural and element-analytical study, Anat. Embryol. 195, 289–297 (1997).
S. R. Grobler, F. S. Theunissen, and T. J. Kotze, The relation between lead concentrations in human dential tissues and in blood, Arch. Oral. Biol. 45, 607–609 (2000).
L. G. Petersson, A. Lodding, and G. Koch, Elemental microanalysis of enamel and dentin by secondary ion mass spectrometry (SIMS), Swed. Dent. J. 2, 41–54 (1978).
D. Kang, D. Amarasiriwardena, and A. H. Goodman, Application of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) to investigate trace metal spatial distributions in human tooth enamel and dentine growth layers and pulp, Anal. Bioanal. Chem. 378, 1608–1615 (2004).
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Arora, M., Chan, S.W.Y., Ryan, C.G. et al. Spatial distribution of lead in enamel and coronal dentine of wistar rats. Biol Trace Elem Res 105, 159–170 (2005). https://doi.org/10.1385/BTER:105:1-3:159
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DOI: https://doi.org/10.1385/BTER:105:1-3:159