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Imaging cell interactions with native and crosslinked polyelectrolyte multilayers

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Abstract

The adhesion of primary chondrocytes to polyelectrolyte multilayer films, made of poly(l-lysine) (PLL) and hyaluronan (HA), was investigated for native and crosslinked films, either ending by PLL or HA. Crosslinking the film was achieved by means of a water-soluble carbodiimide in combination with N-hydroxysulfosuccinimide. The adhesion of macrophages and primary chondrocytes was investigated by microscopical techniques (optical, confocal, and atomic), providing useful information on the cell/film interface. Native films were found to be nonadhesive for the, primary chondrocytes, but could be degraded by macrophages, as could be visualized by confocal laser scanning microscopy after film labeling. Confocal microscopy images show that these films can be deformed by the condrocytes and that PLL diffuses at the chondrocyte membrane. In contrast, the cells adhered and proliferated well on the crosslinked films, which were not degraded by the macrophages. These results were confirmed by a MTT test over a 6-d period and by atomic force microscopy observations. We thus prove that chemical crosslinking can dramatically change cell adhesion properties, the cells being more stably anchored on the crosslinked films.

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Authors and Affiliations

  1. Institut National de la Santé et de la Recherche Médicale, Unité 595, Faculté de Chirurgie Dentaire, Université Louis Pasteur, 11 rue Humann, 67085, Strasbourg Cedex, France

    Ludovic Richert, Aurore Schneider, Dominique Vautier, Constant Vodouhe, Nadia Jessel, Jean-Claude Voegel & Catherine Picart

  2. Laboratoire de Physiopathologie et Pharmacologie Articulaires UMR 7561 et IFR 111, Faculté de Médecine, 54 505, Vandoeuvre-les-Nancy, France

    Elisabeth Payan

  3. Institut Charles Sadron Centre National de la Recherche Scientifique, Université Louis Pasteur, 6 rue Boussingault, 67083, Strasbourg Cedex, France

    Pierre Schaaf

  4. Ecole Européenne de Chimie, Polymères et Matériaux de Strasbourg, 25 rue Becquerel, 67087, Strasbourg Cedex 2, France

    Pierre Schaaf & Catherine Picart

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  1. Ludovic Richert
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  2. Aurore Schneider
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  3. Dominique Vautier
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  4. Constant Vodouhe
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  5. Nadia Jessel
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  6. Elisabeth Payan
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  7. Pierre Schaaf
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  8. Jean-Claude Voegel
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  9. Catherine Picart
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Correspondence to Catherine Picart.

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Richert, L., Schneider, A., Vautier, D. et al. Imaging cell interactions with native and crosslinked polyelectrolyte multilayers. Cell Biochem Biophys 44, 273–285 (2006). https://doi.org/10.1385/CBB:44:2:273

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