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Adverse effects of environmental toxicants, octylphenol and bisphenol A, on male reproductive functions in pubertal rats

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Abstract

It has been proposed that a global decline in sperm counts, semen quality, and several male reproductive disorders are associated with exposure to environmental chemicals. Thus, the present study examined the effects of two estrogenic chemicals, octylphenol (OP) and bisphenol A (BPA), on epididymal sperm counts and sperm motility, luteinizing hormone (LH)-releasing hormone (LHRH)-stimulated plasma LH and steroid hormones, insulin-like growth factor I (IGF-I), and accessory reproductive organs in pubertal male Wistar rats. Fifty-day-old rats in the OP group (n=11) and BPA group (n=11) received daily sc injections of the respective chemical at a dose of 3 mg/kg bw dissolved in 0.2 mL DMSO. Rats in the control group (DMSO group; n=10) received 0.2 mL DMSO alone. After 2 wk of treatment, a jugular blood sample was taken, and, on the next day, a second blood sample was taken 1 h after an sc injection of LHRH (250 ng). After 5 wk of treatment, rats were deeply anesthetized and heart blood was collected. Epididymal sperm motility and sperm head counts were determined. LHRH increased plasma LH to higher levels in all groups, but the increases were significant (p<0.01) in the BPA and OP groups. However, despite higher LH levels after LHRH injection, the incremental responses of testosterone and progesterone in the OP and BPA groups were small compared to those in the DMSO group, which showed a small LH response. After 5 wk of treatment, plasma testosterone levels were significantly (p<0.01) reduced in the OP and BPA groups and this was accompanied by reduced (p<0.05) epididymal sperm counts. However, the chemical-treated groups had high basal progesterone levels. No significant effects of chemicals on sperm motility parameters were noted. The chemical-induced increases (p<0.05) of the weight of ventral prostate gland were coincided with elevated plasma IGF-I levels in the BPA (p<0.05) and OP (p<0.01) groups. The present results demonstrated that OP and BPA can reduce sperm counts resulting from lowered plasma testosterone in male rats just after puberty. The enlarged ventral prostate gland may possibly be associated with increased plasma IGF-I, raising the possibility of a link between these chemicals and prostate diseases because IGF-I has been implicated in the pathogenesis of human prostate cancers.

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Author notes

  1. Chandana B. Herath

    Present address: Department of Medicine, University of Melbourne, Austin and Repatriation Medical Center, 3084, Heidelberg, Victoria, Australia

Authors and Affiliations

  1. United Graduate School of Veterinary Sciences, 501-1193, Gifu

    Wanzhu Jin, Gen Watanabe & Kazuyoshi Taya D.V.M., Ph.D.

  2. Laboratory of Tissue Physiology, Tokyo University of Agriculture and Technology, 305-8509, Tokyo

    Koji Arai

  3. Air Pollutants Health Effects Research Team, National Institute of Environmental Studies, 305-0053, Ibaraki, Japan

    Akira K. Suzuki

  4. Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, 183-8509, Tokyo, Japan

    Chandana B. Herath, Wanzhu Jin, Gen Watanabe & Kazuyoshi Taya D.V.M., Ph.D.

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  1. Chandana B. Herath
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Correspondence to Kazuyoshi Taya D.V.M., Ph.D..

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Herath, C.B., Jin, W., Watanabe, G. et al. Adverse effects of environmental toxicants, octylphenol and bisphenol A, on male reproductive functions in pubertal rats. Endocr 25, 163–172 (2004). https://doi.org/10.1385/ENDO:25:2:163

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  • DOI: https://doi.org/10.1385/ENDO:25:2:163

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