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Universal primers for the PCR-mediated amplification of DNA β

A molecule associated with some monopartite begomoviruses

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Abstract

DNA β is an approx 1350 nucleotide, single-stranded DNA molecule which has been shown to be associated with some monopartite geminiviruses of the genus Begomovirus. This component requires the helper begomovirus for replication in the cells of host plants and for insect transmission, possibly by trans-encapsidation. Sequence comparisons of the two available DNA β sequences has identified a highly conserved region upstream of a predicted hairpin structure. Abutting primers designed to this conserved region allows PCR-mediated amplification of the full-length DNA β component from total nucleic acid extracts isolated from infected plants originating from a variety of geographically distinct sources and host plants.

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References

  1. Rybicki, E. P., Briddon, R. W., Brown, J. E., et al. (2000) Geminiviridae, in “Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses” (van Regenmortel, M. H. V., Fauquet, C. M., Bishop, D. H. L., Carstens, E. B., Estes, M. K., Lemon, S. M., Maniloff, J., Mayo, M. A., McGeoch, D. J., Pringle, C. R., and Wicker, R. B., eds.), pp. 285–297. Academic Press, San Diego.

    Google Scholar 

  2. Navot, N., Pichersky, E., Zeidan, M., Zamir, D., and Czosnek, H. (1991) Tomato yellow leaf curl virus: a whitefly-transmitted geminivirus with a single genomic component. Virology 185, 151–161.

    Article  PubMed  CAS  Google Scholar 

  3. Kheyr-Pour, A., Bendahmane, M., Matzeit, V., Accotto, G. P., Crespi, S., and Gronenborn, B. (1992) Tomato yellow leaf curl virus from Sardinia is a whitefly- transmitted monopartite geminivirus. Nucleic Acids Res. 19, 6763–6769.

    Article  Google Scholar 

  4. Czosnek, H. and Laterrot, H. (1997) A worldwide survey of tomato yellow leaf curl viruses. Arch. Virol. 142, 1391–1406.

    Article  PubMed  CAS  Google Scholar 

  5. Polston, J. E., McGovern, R. J., and Brown, L. G. (1999) Introduction of tomato yellow leaf curl virus in Florida and implications for the spread of this and other geminiviruses of tomato. Plant Dis. 83, 984–988.

    Article  Google Scholar 

  6. McGlashan, D., Polston, J. E., and Bois, D. (1994) Tomato yellow leaf curl virus in Jamaica. Plant Dis. 78, 1219.

    Article  Google Scholar 

  7. Tan, P. H. N., Wong, S. M., Wu, M., Bedford, I. D., Saunders, K., and Stanley, J. (1995) Genome organization of ageratum yellow vein virus, a monopartite whitefly-transmitted geminivirus isolated from a common weed. J. Gen. Virol. 76, 2915–2922.

    Article  PubMed  CAS  Google Scholar 

  8. Briddon, R. W., Mansoor, S., Bedford, I. D., Pinner, M. S., and Markham, P. G. (2000) Clones of cotton leaf curl geminivirus induce symptoms atypical of cotton leaf curl disease. Virus Genes 20, 17–24.

    Article  Google Scholar 

  9. Mansoor, S., Khan, S. H., Bashir, A., et al. G. (1999) Identification of a novel circular single-stranded DNA associated with cotton leaf curl disease in Pakistan. Virology 259, 190–199.

    Article  PubMed  CAS  Google Scholar 

  10. Saunders, K. and Stanley, J. (1999) A nanovirus-like component associated with yellow vein disease of Ageratum yellow vein disease of Ageratum conyzoides: evidence for interfamilial recombination between plant disease plant DNA viruses. Virology 264, 142–152.

    Article  PubMed  CAS  Google Scholar 

  11. Saunders, K., Bedford, I. D., Briddon, R. W., Markham, P. G., Wong, S. M., and Stanley, J. (2000) A novel virus complex causes Ageratum yellow vein disease. Proc. Natl. Acad. Sci. USA 97, 6890–6895.

    Article  PubMed  CAS  Google Scholar 

  12. Briddon, R., Mansoor, S., Bedford, I., et al. (2001) Identification of DNA components required for induction of cotton leaf curl disease. Virology, 285, 234–243.

    Article  PubMed  CAS  Google Scholar 

  13. Laufs, J., Traut, W., Heyraud, F., et al. (1995) In vitro cleavage and joining at the viral origin of replication by the replication initiator protein of tomato yellow leaf curl virus. Proc. Natl. Acad. Sci. USA 92, 3879–3883.

    Article  PubMed  CAS  Google Scholar 

  14. Doyle, J. J. and Doyle, J. L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf material. Phytochem. Bull. 19, 11–15.

    Google Scholar 

  15. Covey, S. N. and Hull, R. (1981) Transcription of cauliflower mosaic virus DNA. Detection of transcripts, properties and location of the gene encoding the virus inclusion body protein. Virology 111, 463–474.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Disease and Stress Biology, John Innes Centre, Colney Lane, NR4 7UH, Norwich, UK

    R. W. Briddon

  2. National Institute of Biotechnology and Genetic Engineering, Jhang Road, P.O. Box 577, Faisalabad, Pakistan

    S. E. Bull, S. Mansoor & I. Amin

Authors
  1. R. W. Briddon
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  2. S. E. Bull
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  3. S. Mansoor
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  4. I. Amin
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  5. P. G. Markham
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Correspondence to R. W. Briddon.

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Briddon, R.W., Bull, S.E., Mansoor, S. et al. Universal primers for the PCR-mediated amplification of DNA β. Mol Biotechnol 20, 315–318 (2002). https://doi.org/10.1385/MB:20:3:315

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  • DOI: https://doi.org/10.1385/MB:20:3:315

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