Abstract
Restriction digestion is routinely performed in a buffer compatible with the restriction enzyme used. To load the samples on agarose gels for electrophoresis it is then necessary to add a loading buffer. A 10X loading buffer is often used, and consists of a dye to track the electrophoresis and a dense solution so that the digestion mixture sinks into the well. We describe a new buffer, which acts as a universal digestion buffer as well as a loading buffer. This avoids double handling of samples, which wastes both time and consumables. Importantly, the efficiency of digestion was found not to be significantly decreased in this new buffer.
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Corbett, A.J., Brady, J.L. & Lew, A.M. A single buffer that universally serves both restriction digestion and loading. Mol Biotechnol 21, 51–55 (2002). https://doi.org/10.1385/MB:21:1:051
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DOI: https://doi.org/10.1385/MB:21:1:051