In a rapid serial visual presentation (RSVP) task, the processing of a preceding target (T1) interrupts the response to a subsequent target (T2). This phenomenon is thought to indicate the temporal limitations of attention and is named attentional blink (AB). In previous studies two conflicting models have been proposed for the AB phenomenon: the serial processing model and the parallel processing model. The present study examined which could be more appropriate. We used negativity bias, which is a phenomenon that negative stimuli are more rapidly processed because they attract more attention than neutral stimuli. When T2 was negative the AB deficit decreased (Experiment 1). However, when T1 was negative the AB deficit increased (Experiment 2). Moreover, when both T1 and T2 were negative the AB deficits observed in Experiment 1 and Experiment 2 canceled each other (Experiment 3). It was therefore considered that the AB was due to attentional interference during parallel processing of RSVP items.