Interphase nuclear structure and heterochromatin were studied in Corchorus olitorius, Corchorus capsularis and their F₁ hybrid by HCl-Giemsa and BSG-technique. Nuclear structure was chromocentric in both the parents and the hybrid. Chromocentre numbers in two species of Corchorus and their hybrid were more or less same, which were, however, found to reduce than the expected number of 14. Heterochromatin values yielded by HCl-Giemsa technique were slightly higher than those yielded by BSG treatment. The percentage of heterochromatin in F₁ was found to be closer to the male parent. DNA contents were also estimated in both the parents and their hybrid but the relationship between DNA contents and heterochromatin value was not clear.