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Isolation and expression analyses of KLUH gene in developing seeds and enhanced seed oil in KLUH overexpressing Brassica juncea transgenics

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Abstract

Improving oil yield of Indian mustard (Brassica juncea) is exigent as it is a major oilseed crop of Indian subcontinent, which has severe shortage of vegetable oil production in the world. Some of the regulators of seed development have been shown to improve oil yield in Arabidopsis. Arabidopsis KLUH (AtKLUH), a maternal regulator of seed size, has been shown to control seed oil content. In this study, we identified three homologs of AtKLUH in B. juncea, BjKLUH1, BjKLUH2-1 and BjKLUH2-2. We observed that BjKLUH1 differentially expresses in developing seeds in B. juncea accessions with varying seed size and oil content. Further, analyses for seed oil content in B. juncea transgenics carrying AtKLUH demonstrated an increase in seed oil up to 8.3% compared to wild-type plants. The results of this study suggest that KLUH may have a role in seed development and is a good candidate for engineering seed oil accumulation in B. juncea.

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Abbreviations

ARF2:

auxin response factor2

bp:

base pairs

CDS:

coding sequence

DAP:

days after pollination

FA:

fatty acid

GAPDH:

glyceraldehyde-3-phosphate dehydrogenase

nptII:

neomycin phosphotransferase II

ORF:

open reading frame

PCR:

polymerase chain reaction

qRT-PCR:

quantitative reverse-transcription PCR

QTL:

quantitative trait locus

TAG:

triacylglycerol

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Authors and Affiliations

  1. ICAR — Indian Institute of Wheat and Barley Research, Regional station, Flowerdale, Shimla, 171002, India

    Siddanna Savadi

  2. Indian Agricultural Research Institute, ICAR — National Research Centre on Plant Biotechnology, New Delhi -, 110012, India

    Deepak Singh Bisht & Shripad Ramachandra Bhat

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  1. Siddanna Savadi
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  2. Deepak Singh Bisht
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  3. Shripad Ramachandra Bhat
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Correspondence to Siddanna Savadi.

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Savadi, S., Bisht, D.S. & Bhat, S.R. Isolation and expression analyses of KLUH gene in developing seeds and enhanced seed oil in KLUH overexpressing Brassica juncea transgenics. Biologia 72, 1023–1030 (2017). https://doi.org/10.1515/biolog-2017-0123

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