Abstract
Background
Arginine family amino acids (AFAAs) include glutamine (Gln) plus glutamate (Glu), ornithine (Orn), proline (Pro), citrulline (Cit) and arginine (Arg). We aimed to quantitate these amino acids in the blood of full-term infants in relation to their birth weight (BW) perinatally.
Methods
Breastfeeding full-term infants (n = 2000, 1000 males, 1000 females) with a BW of 2000–4000 g were divided into four equal groups: group A, 2000–2500 g; B, 2500–3000 g; C, 3000–3500 g and D, 3500–4000 g. Blood samples as dried blood spots (DBS) were collected on the third day of life and analyzed via a liquid chromatography tandem mass spectrometry (LC-MS/MS) protocol.
Results
Gln plus Glu mean values were found to be statistically significantly different between males and females in all studied groups. The highest values of these amino acids were detected in both males and females in group D. Orn mean values were found to be statistically significantly different between males and females of the same BW in all groups except the last one. The lower mean value was determined in group A, whereas the highest was determined in group D. Cit and Arg mean values were determined to be almost similar in all studied groups.
Conclusions
Gln plus Glu and Orn blood concentrations were directly related to infants’ BW. Conversely, Cit and Arg did not vary significantly in all groups.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: None declared.
References
1. Wu G, Bazer FW, Davis TA, Jaeger LA, Johnson JA, et al. Important roles for the arginine family of amino acids in swine nutrition and production. Livest Sci 2007;112:8–22.10.1016/j.livsci.2007.07.003Search in Google Scholar
2. Wu G. Functional amino acids in nutrition and health. Amino Acids 2013;45:407–11.10.1007/s00726-013-1500-6Search in Google Scholar
3. Wu G, Morris Jr SM. Arginine metabolism: nitric oxide and beyond. Biochem J 1998;336:1–17.10.1042/bj3360001Search in Google Scholar
4. Davis TA, Fiorotto ML, Reeds PJ. Amino acid compositions of body and milk protein change during the suckling period in rats. J Nutr 1993;123:947–56.10.1093/jn/123.5.947Search in Google Scholar
5. Wu G, Davis PK, Flynn NE, Knabe DA, Davidson JT. Endogenous synthesis of arginine plays an important role in maintaining arginine homeostasis in postweaning growing pigs. J Nutr 1997;127:2342–9.10.1093/jn/127.12.2342Search in Google Scholar
6. Flynn NE, Wu G. An important role for endogenous synthesis of arginine in maintaining arginine homeostasis in neonatal pigs. Am J Physiol 1996;271:R1149–55.10.1152/ajpregu.1996.271.5.R1149Search in Google Scholar
7. Bertolo RF, Burrin DG. Comparative aspects of tissue glutamine and proline metabolism. J Nutr 2008;138:2032S–9S.10.1093/jn/138.10.2032SSearch in Google Scholar
8. Regnault TR, de Vrijer B, Battaglia FC. Transport and metabolism of amino acids in placenta. Endocrine 2002;19:23–41.10.1385/ENDO:19:1:23Search in Google Scholar
9. Wu G, Bazer FW, Satterfield MC, Li X, Wang X, et al. Impacts of arginine nutrition on embryonic and fetal development in mammals. Amino Acids 2013;45:241–56.10.1007/s00726-013-1515-zSearch in Google Scholar PubMed
10. Wu G, Bazer FW, Johnson GA, Herring C, Seo H, et al. Functional amino acids in the development of the pig placenta. Mol Reprod Dev 2017;84:870–82.10.1002/mrd.22809Search in Google Scholar PubMed
11. Ivorra C, Garcia-Vicent C, Chaves FJ, Monleón D, Morales JM, et al. Metabolomic profiling in blood from umbilical cords of low birth weight newborns. J Transl Med 2012;10:142.10.1186/1479-5876-10-142Search in Google Scholar PubMed PubMed Central
12. Lin G, Wang XQ, Wu GY, Feng CP, Zhou HJ, et al. Improving amino acid nutrition to prevent intrauterine growth, restriction in mammals. Amino Acids 2014;46:1605–23.10.1007/s00726-014-1725-zSearch in Google Scholar PubMed
13. Hurwitz R, Kretchmer N. Development of arginine-synthesizing enzymes in mouse intestine. Am J Physiol 1986;251:G103–10.10.1152/ajpgi.1986.251.1.G103Search in Google Scholar PubMed
14. De Jonge WJ, Dingemanse MA, de Boer PA, Lamers WH, Moorman AF. Arginine-metabolizing enzymes in the developing rat small intestine. Pediatr Res 1998;43:442–51.10.1203/00006450-199804000-00002Search in Google Scholar PubMed
15. Wu G. Synthesis of citrulline and arginine from proline in enterocytes of postnatal pigs. Am J Physiol 1997;272:G1382–90.10.1152/ajpgi.1997.272.6.G1382Search in Google Scholar PubMed
16. Wu G, Knabe DA. Arginine synthesis in enterocytes of neonatal pigs. Am J Physiol 1995;269:R621–9.10.1152/ajpregu.1995.269.3.R621Search in Google Scholar PubMed
17. Wu G, Knabe DA, Flynn NE. Synthesis of citrulline from glutamine in pig enterocytes. Biochem J 1994;299:115–21.10.1042/bj2990115Search in Google Scholar PubMed PubMed Central
18. Kohler ES, Sankaranarayanan S, Van Ginneken CJ, Van Dijk P, Vermeulen JL, et al. The human neonatal small intestine has the potential for arginine synthesis; developmental changes in the expression of arginine-synthesizing and -catabolizing enzymes. BMC Dev Biol 2008;8:107.10.1186/1471-213X-8-107Search in Google Scholar PubMed PubMed Central
19. Davis TA, Nguyen HV, Garcia-Bravo R, Fiorotto ML, Jackson EM, et al. Amino acid composition of human milk is not unique. J Nutr 1994;124:1126–32.10.1093/jn/124.7.1126Search in Google Scholar PubMed
20. Zhang Z, Adelman AS, Rai D, Boettcher J, Lőnnerdal B. Amino acid profiles in term and preterm human milk through lactation: a systematic review. Nutrients 2013;5:4800–21.10.3390/nu5124800Search in Google Scholar PubMed PubMed Central
21. Chuang CK, Lin SP, Lee HC, Wang TJ, Shih YS, et al. Free amino acids in full-term and pre-term human milk and infant formula. J Pediatr Gastroenterol Nutr 2005;40:496–500.10.1097/01.MPG.0000150407.30058.47Search in Google Scholar
22. Li P, Knabe DA, Kim SW, Lynch CJ, Hutson SM, et al. Lactating porcine mammary tissue catabolizes branched-chain amino acids for glutamine and aspartate synthesis. J Nutr 2009;139:1502–9.10.3945/jn.109.105957Search in Google Scholar PubMed PubMed Central
23. Xi PB, Jiang ZY, Zheng CT, Lin Y, Wu G. Regulation of protein metabolism by glutamine: implications for nutrition and health. Front Biosci 2011;16:578–97.10.2741/3707Search in Google Scholar PubMed
24. Shah PS, Shah VS, Kelly LE. Arginine supplementation for prevention of necrotising enterocolitis in preterm infants. Cochrane Database Syst Rev 2017;11:CD004339.10.1002/14651858.CD004339.pub4Search in Google Scholar PubMed PubMed Central
25. Sánchez CL, Cubero J, Sánchez J, Franco L, Rodríguez AB, et al. Evolution of the circadian profile of human milk amino acids during breastfeeding. J Appl Biomed 2013;11: 59–70.10.2478/v10136-012-0020-0Search in Google Scholar
26. Yang L, Zhang Y, Yang J, Huang X. Effects of birth weight on profiles of dried blood amino-acids and acylcarnitines. Ann Clin Biochem 2018;55:92–9.10.1177/0004563216688038Search in Google Scholar PubMed
27. Yang Y, Yu B, Long W, Wang H, Wang Y, et al. Investigating the changes in amino acid values in premature infants: a pilot study. J Pediatr Endocrinol Metab 2018;31:435–41.10.1515/jpem-2017-0372Search in Google Scholar PubMed
28. Loukas YL, Soumelas GS, Dotsikas Y, Georgiou V, Molou E, et al. Expanded newborn screening in Greece: 30 months of experience. J Inherit Metab Dis 2010;33:S341–8.10.1007/s10545-010-9181-8Search in Google Scholar PubMed
29. Svanberg U, Gebre-Medhin M, Ljungqvist B, Olsson M. Breast milk composition in Ethiopian and Swedish mothers. III. Amino acids and other nitrogenous substances. Am J Clin Nutr 1977;30:499–507.10.1093/ajcn/30.4.499Search in Google Scholar PubMed
30. Dearden L, Bouret SG, Ozanne SE. Sex and gender differences in developmental programming of metabolism. Mol Metab 2018;15:8–19.10.1016/j.molmet.2018.04.007Search in Google Scholar PubMed PubMed Central
31. Day PE, Ntani G, Crozier SR, Mahon PA, Inskip HM, et al. Maternal factors are associated with the expression of placental genes involved in amino acid metabolism and transport. PLoS One 2015;10:e0143653.10.1371/journal.pone.0143653Search in Google Scholar PubMed PubMed Central
32. Wu X, Xie C, Zhang Y, Fan Z, Yin Y, et al. Glutamate–glutamine cycle and exchange in the placenta–fetus unit during late pregnancy. Amino Acids 2015;47:45–53.10.1007/s00726-014-1861-5Search in Google Scholar PubMed
33. Manta-Vogli PD, Schulpis KH, Dotsikas Y, Loukas YL. The significant role of amino acids during pregnancy: nutritional support. J Matern Fetal Neonatal Med 2018:1–7. doi: 10.1080/14767058.2018.1489795 [Epub ahead of print].10.1080/14767058.2018.1489795Search in Google Scholar PubMed
34. Schulpis KH, Vlachos GD, Papakonstantinou ED, Karikas GA, Vlachos DG, et al. Maternal-neonatal amino acid blood levels in relation to the mode of delivery. Acta Obstet Gynecol Scand 2009;88:71–6.10.1080/00016340802578098Search in Google Scholar PubMed
35. Wang B, Wu Z, Ji Y, Sun K, Dai Z, et al. l-Glutamine enhances tight junction integrity by activating CaMK kinase 2-AMP-activated protein kinase signaling in intestinal porcine epithelial cells. J Nutr 2016;146:501–8.10.3945/jn.115.224857Search in Google Scholar PubMed
36. Suryawan A, Hawes JW, Harris RA, Shimomura Y, Jenkins AE, et al. A molecular model of human branched-chain amino acid metabolism. Am J Clin Nutr 1998;68:72–81.10.1093/ajcn/68.1.72Search in Google Scholar PubMed
37. Che L, Yang Z, Xu M, Xu S, Che L, et al. Maternal nutrition modulates fetal development by inducing placental efficiency changes in gilts. BMC Genomics 2017;18:213.10.1186/s12864-017-3601-1Search in Google Scholar PubMed PubMed Central
38. Collado MC, Santaella M, Mira-Pascual L, Martinez-Arias E, Khodayar-Pardo P, et al. Longitudinal study of cytokine expression, lipid profile and neuronal growth factors in human breast milk from term and preterm. Nutrients 2015;19:8577–91.10.3390/nu7105415Search in Google Scholar PubMed PubMed Central
39. Blachier F, M’Rabet-Touil H, Posho L, Darcy-Vrillon B, Duée PH. Intestinal arginine metabolism during development. Evidence for de novo synthesis of L-arginine in newborn pig enterocytes. Eur J Biochem 1993;216:109–17.10.1111/j.1432-1033.1993.tb18122.xSearch in Google Scholar PubMed
40. Ginguay A, Cynober L, Emmanuel Curis E, Nicolis I. Ornithine aminotransferase, an important glutamate-metabolizing enzyme at the cross roads of multiple metabolic pathways. Biology (Basel) 2017;6. pii: E18. doi: 10.3390/biology6010018.Search in Google Scholar PubMed PubMed Central
41. Ioannou HP, Diamanti E, Piretzi K, Drossou-Agakidou V, Augoustides-Savvopoulou P. Plasma citrulline levels in preterm neonates with necrotizing enterocolitis. Early Hum Dev 2012;88:563–6.10.1016/j.earlhumdev.2011.11.008Search in Google Scholar PubMed
42. Crenn P, Coudray-Lucas C, Thuillier F, Cynober L, Messing B. Postabsorptive plasma citrulline concentration is a marker of absorptive enterocyte mass and intestinal failure in humans. Gastroenterology 2000;119:1496–505.10.1053/gast.2000.20227Search in Google Scholar PubMed
43. Tharakan JF, Yu YM, Zurakowski D, Roth RM, Young VR, et al. Adaptation to a long term (4 weeks) arginine- and precursor (glutamate, proline and aspartate)-free diet. Clin Nutr 2008;27:513–22.10.1016/j.clnu.2008.04.014Search in Google Scholar PubMed PubMed Central
44. Wilkinson DL, Bertolo RF, Brunton JA, Shoveller AK, Pencharz PB, et al. Arginine synthesis is regulated by dietary arginine intake in the enterally fed neonatal piglet. Am J Physiol Endocrinol Metab. 2004;287:E454–62.10.1152/ajpendo.00342.2003Search in Google Scholar PubMed
45. Hou Z-P, Yin Y-L, Huang R-L, Li T-J, Hou R, et al. Rice protein concentrate partially replaces dried whey in the diet for early-weaned piglets and improves their growth performance. J Sci Food Agric 2008;88:1187–93.10.1002/jsfa.3196Search in Google Scholar
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