Abstract
Antiserum against crystallized 20β-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was used for different immunodiffusion and immunoprecipitation tests to quantify the bacterial enzyme in cell-free supernatants of the microorganism. After immunoprecipitation and gel electrophoresis the molecular weight of the subunits of 20β-hydroxysteroid dehydrogenase was calculated to be 27 300 ± 700.
Keywords: 20β-Hydroxysteroid Dehydrogenase; Immunoprecipitation; Immunodiffusion; Molecular Weight; Streptomyces hydrogenans
Received: 1976-1-30
Published Online: 2014-6-2
Published in Print: 1976-6-1
© 1946 – 2014: Verlag der Zeitschrift für Naturforschung
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