Abstract
Yeast cell wall matrix particles are composed entirely of β-glucan and mannoprotein. Alkali-insoluble (1→3)-β-d-glucan was extracted from the yeast cell wall by an alkaline-acid method. IR spectra analysis showed that the product was chemically pure glucan, that is to say, it contained no other carbohydrates and proteins. So, the alkaline-acid method was ideal for extracting (1→3)-β- d-glucan from the yeast cell wall. We also purified and analyzed mannan oligosaccharides by the dilute alkali- Sevage method from the yeast cell wall.
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