Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Cellular Protective Effect and Liposome Formulation for Enhanced Transdermal Delivery of Isoquercitrin

Isoquercitrin의 세포 보호 작용과 피부 흡수 증진을 위한 리포좀 제형 연구

  • Jo, Na-Rae (Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • Gu, Hyun-A (Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • Park, Su-Ah (Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • Han, Seat-Byeol (Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • Park, Soo-Nam (Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D center, Seoul National University of Science and Technology)
  • 조나래 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실 화장품종합기술연구소) ;
  • 구현아 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실 화장품종합기술연구소) ;
  • 박수아 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실 화장품종합기술연구소) ;
  • 한샛별 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실 화장품종합기술연구소) ;
  • 박수남 (서울과학기술대학교 정밀화학과 나노바이오화장품연구실 화장품종합기술연구소)
  • Received : 2012.03.29
  • Accepted : 2012.06.12
  • Published : 2012.06.30
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Abstract

In this study, the cellular protective effect of isoquercitrin against $H_2O_2$ and rose bengal-indued HaCaT cell damage was investigated. The ethosome and elastic liposome for enhanced transdermal delivery were prepared. Particle size, loading efficiency and cumulative permeated amounts of them were evaluated. Isoquercitrin didn't show any characteristic cytotoxicity at 50 ${\mu}M$. When HaCaT cells were treated with 5 mM $H_2O_2$ and 25 ${\mu}M$ rose bengal, isoquercitrin protected the cells against the oxidative damage in a concentration dependent manner (6.25 ~ 50 ${\mu}M$). The size of 0.03 % isoquercitrin loaded ethosome was 222.85 nm and the loading efficiency was 82.26 %. The ethosome loaded with 0.03 % isoquercitrin was stable and maintained the constant particle size for 2 weeks after being prepared. The ethosome exhibited more enhanced skin permeability than general liposome and ethanol solution. The optimal ratio of lipid to surfactant of 0.1 % isoquercitrin loaded elastic liposomes was observed to be 89 : 5 through evaluating particle size (341.2 nm), deformability index (59.89), loading efficiency (54.3 %), and skin permeability (54.4 %).

본 연구에서는 $H_2O_2$와 rose bengal로 처리된 HaCaT 세포에 있어서 isoquercitrin의 세포 보호 효과를 조사하였다. Isoquercitrin의 피부 전달시스템으로 에토좀 및 탄성 리포좀을 제조하고 입자크기, 포집효율 및 피부 흡수 증진 능력을 평가하였다. Isoquercitrin은 HaCaT 세포에 대해 50 ${\mu}M$의 농도에서 독성을 나타내지 않았다. 5 mM의 $H_2O_2$ 및 25 ${\mu}M$의 rose bengal로 HaCaT 세포를 처리하였을 때 isoquercitrin은 산화적 손상에 대항하여 농도 의존적(6.25 ~ 50 ${\mu}M$)으로 세포 보호 효과를 나타내었다. 0.03 % Isoquercitrin을 담지한 에토좀의 입자 크기는 222.85 nm, 포집효율은 82.26 %였다. 0.03 % isoquercitrin 함유 에토좀은 제조 후 2주일 동안 안정하였고, 일정한 입자 크기를 유지하였다. 피부 투과 실험 결과 에토좀은 일반 리포좀이나 에탄올 용액에서 보다 우수한 피부 투과능을 보여주었다. 0.1 % Isoquercitrin을 담지한 탄성 리포좀의 최적의 제형은 입자 크기(341.2 nm), 가변형성(59.89), 포집효율(54.3 %) 및 피부투과능 (초기 적하량 대비 54.4 %) 확인을 통해 인지질 대 계면활성제의 비율이 85 : 15인 제형이 가장 우수한 탄성 리포좀 제형임을 나타내었다.

Keywords

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