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Electrochemical Release of Immobilized IgG Protein

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Abstract

In this paper, we present the electrochemically programmed release of immobilized IgG protein molecules that have been attached to gold coated surfaces via a thiol-gold linkage. Fluorescence microscopy has been used to image the release of fluorescently tagged IgGs in phosphate buffered saline. In this technique, the reductive desorption of self-assembled monolayers is employed for the release of proteins, which are immobilized on the surface either by non-covalent or covalent interactions. The voltage applied for the release of proteins is in a range of -1.5V to -60V.

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References

  1. R.G. Nuzzo and D.L. Allara, “Adsorption of bifunctional organic disulfides on gold surfaces”, Journal of American Chemical Society, vol. 105, pp. 4481–4483, 1983.

    Article  CAS  Google Scholar 

  2. M.D. Porter, T.B. Bright, D.L. Allara, C.E.D. Chidseyi, “Spontaneously organized molecular assemblies .4. structural characterization of n-alkyl thiol monolayers on gold by optical ellipsometry, infrared spectroscopy, and electrochemistry”, Journal of American Chemical Society, vol. 109, pp. 3559–3568, 1987.

    Article  CAS  Google Scholar 

  3. P. Mali, N. Bhattacharjee, and P.C. Searson, “Electrochemically programmed release of biomolecules and nanoparticles”, Nano Letters, vol. 6, pp. 1250–1253, 2006.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. School of Electrical and Computer Engineering, RMIT University, Melbourne, 3001, Australia

    Tanveer Mahmud, Wojtek Wlodarski, Arnan Mitchell & Kourosh Kalantar-zadeh

  2. Department of Chemical and Biomolecular Engineering, and The Bio21 Molecular Science & Biotechnology, The University of Melbourne, Melbourne, 3004, Australia

    Sally Gras

  3. Manufacturing and Materials Technology, CSIRO, Melbourne, Australia

    Adrian Trinchi

Authors
  1. Tanveer Mahmud
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  2. Wojtek Wlodarski
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  3. Arnan Mitchell
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  4. Sally Gras
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  5. Adrian Trinchi
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  6. Kourosh Kalantar-zadeh
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Mahmud, T., Wlodarski, W., Mitchell, A. et al. Electrochemical Release of Immobilized IgG Protein. MRS Online Proceedings Library 1010, 601 (2007). https://doi.org/10.1557/PROC-1010-V06-01

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  • DOI: https://doi.org/10.1557/PROC-1010-V06-01

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