Imunoglobulinas do líquido cefalorraqueano normal

Livramento, J. A.

doi:10.1590/S0004-282X1979000300002

Resumos

São revistos os principais métodos e resultados encontrados na literatura para as imunoglobulinas do líquido cefalorraqueano normal. Foram estudadas as imunoglobulinas do LCR de 50 pessoas com cefaléia crônica apresentando exame físico e neurológico normais bem como eletrencefalograma, exame do LCR por punção sub-occipital e proteinograma do soro dentro dos limites da normalidade. O estudo das imunoglobulinas foi realizzado pelo método de imunodifusão radial. Os resultados obtidos quanto as imunoglobulinas do LCR variaram entre 1,55 e 3,97 mg/100 ml ou 0,17 e 0,44 UI para a IGG; para IGA os valores encontravam-se entre 0 e 0,62 mg/100 ml ou 0 e 0,37 UI. Em todos os casos não foi detectada a presença de IGM no LCR. Na análise dos resultados foi concluído que para o LCR da cisterna magna em pessoas normais a imuno-glubulina IGG é encontrada sempre com concentração média de 2,37 mg/100 ml e desvio padrão de 0,59. A imunoglobulina IGA é encontrada na maioria das ocasiões com concentração média de 0,34 mg/100 ml e desvio padrão de 0,17. A imunoglobulina IGM não é demonstrada em condições normais pelo método utilizado.

The most important methods and results found in literature on immunoglobulins of the normal cerebrospinal fluid (CSF) are reviewed. A study was made of immunoglobulins in the CSF of 50 individuals who suffered from chronic migraine but showed normal results for the physical and neurological examinations; electroencephalograph, CSF tests (sub-occipital puncture) and proteinogram of the serum also fell within the limits of normalcy in all of the 50 cases. The study of immunoglobulins was conducted through the radial immunodifusion method. Results obtained as to immunoglobulins of the CSF varied between 1,55 and 3,97 mg/100 ml, or 0,17 and 0,44 for IGG; values lay between 0 and 0,62 mg/100 ml, or 0,37 UI for IGA. Presence of IGM was not detected in any of the cases studied. Analysis of the results showed that, for the CSF of the cisterna magna in normal individuals, IGG is always found at an average concentration of 2,37 mg/100 ml and a standard deviation of 0,59. IGA is mostly found at an average concentration of 0,34 mg/100 ml and a standard deviation of 0,17. IGM is not demonstrable, under nomal conditions, by the method chosen by the author.

Imunoglobulinas do líquido cefalorraqueano normal

Cerebrospinal fluid immunoglobulins: normal values

J. A. Livramento

Médico Assistente do Centro de Investigações em Neurologia

RESUMO

São revistos os principais métodos e resultados encontrados na literatura para as imunoglobulinas do líquido cefalorraqueano normal. Foram estudadas as imunoglobulinas do LCR de 50 pessoas com cefaléia crônica apresentando exame físico e neurológico normais bem como eletrencefalograma, exame do LCR por punção sub-occipital e proteinograma do soro dentro dos limites da normalidade. O estudo das imunoglobulinas foi realizzado pelo método de imunodifusão radial.

Os resultados obtidos quanto as imunoglobulinas do LCR variaram entre 1,55 e 3,97 mg/100 ml ou 0,17 e 0,44 UI para a IGG; para IGA os valores encontravam-se entre 0 e 0,62 mg/100 ml ou 0 e 0,37 UI. Em todos os casos não foi detectada a presença de IGM no LCR. Na análise dos resultados foi concluído que para o LCR da cisterna magna em pessoas normais a imuno-glubulina IGG é encontrada sempre com concentração média de 2,37 mg/100 ml e desvio padrão de 0,59. A imunoglobulina IGA é encontrada na maioria das ocasiões com concentração média de 0,34 mg/100 ml e desvio padrão de 0,17. A imunoglobulina IGM não é demonstrada em condições normais pelo método utilizado.

SUMMARY

The most important methods and results found in literature on immunoglobulins of the normal cerebrospinal fluid (CSF) are reviewed. A study was made of immunoglobulins in the CSF of 50 individuals who suffered from chronic migraine but showed normal results for the physical and neurological examinations; electroencephalograph, CSF tests (sub-occipital puncture) and proteinogram of the serum also fell within the limits of normalcy in all of the 50 cases. The study of immunoglobulins was conducted through the radial immunodifusion method.

Results obtained as to immunoglobulins of the CSF varied between 1,55 and 3,97 mg/100 ml, or 0,17 and 0,44 for IGG; values lay between 0 and 0,62 mg/100 ml, or 0,37 UI for IGA. Presence of IGM was not detected in any of the cases studied. Analysis of the results showed that, for the CSF of the cisterna magna in normal individuals, IGG is always found at an average concentration of 2,37 mg/100 ml and a standard deviation of 0,59. IGA is mostly found at an average concentration of 0,34 mg/100 ml and a standard deviation of 0,17. IGM is not demonstrable, under nomal conditions, by the method chosen by the author.

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Resumo de Dissertação de Mestrado da Clínica Neurológica da Faculdade de Medicina da Universidade de São Paulo.

Centro de Investigações em Neurologia, FMUSP - Caixa Postal S199 - 0X000 São Paulo, SP - Brasil.

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Publicação nesta coleção
21 Ago 2012
Data do Fascículo
Set 1979

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[1] 1. ANDERSON, S. G.; BANGHAM, D. R.; BATTY, I.: BECKER, W.; CINADER, B.; VAN DER GIESSEN, M.; HYMES, F. LONG, D.; PEETOOM, F. PONDMAN, K.; RAYNAUD, M.; REIMER, C. B.; ROWE, D. S.; SCHWICK, H. G. & VRAN-CHEVA, S. - Measurements of concentrations of human serum immunoglobulins. J. clin. Path. 25:133, 1972.

[2] 2. BAUER,. H. & GOTTESLEBEN, A. - Quantitative immunochemical studies of cerebrospinal fluid proteins in relation to clinical activity of multiple sclerosis. Int. Arch. Allergy 36 (suppl.): 643, 1969.

[3] 3. BERNER, J. J.; CIEMINS, V. A. & SCHROEDER Jr., E. F. - Radial immunodiffusion of spinal fluid: diagnostic value in multiple sclerosis. Amer, J. clin. Path. 58:145, 1972.

[4] 4. BOCK, E. - Quantitation of plasma proteins in cerebrospinal fluid. Scand. J. Immunol. 2 (suppl. 2 (suppl. 1):111, 1973.

[5] 5. BOCK, E., KRISTENSEN, V. & RAFAELSEN, O. J. - Proteins in serum ana cerebrospinal fluid in dement patients. Acta neurol. scand. 50:91, 1974.

[6] 6. BROOKS, R. A. - Quantitation of cerebrospinal fluid proteins. Amer. J. clin. Path. 59:886, 1973.

[7] 7. BUCKLEY III, C. E. & DORSEY, F. C. - The effect of aging on human serum immunoglobulin concentrations. J. Immunol. 105:964, 1970.

[8] 8. BUCKLEY III, C. E. & DORSEY, F. C. - Serum immunoglobulin levels throughout the life-span of healthy man. Ann. intern. Med. 75:673, 1971.

[9] 9. CAZZULLO, C. L._; MONTANINI, R.; PALTRINIERI, E. & GIOVANNUCCI, M. - II comportamento delle immunoglobuline nella sclerosi múltipla. Acta neurol. (Napoli) 23:589, 1968.

[10] 10. CERRATO, D.; CORRIDORI, F. & NESPOLO, A. - L'immunodiffusione radiale semplice sul liquor: metódica e sue applicazioni. Riv. Patol. nerv. ment. 95:572, 1974.

[11] 11. CHODIRKER, W. B. & TOMASI Jr., T. B. - Gamma globulins: quantitative relationships in human serum and non vascular fluids. Science 112:1080, 1963.

[12] 12. CLAUSEN, J.; FOG, T. & EINSTEIN, E. R. - The clinical value of assaying proteins in the cerebrospinal fluid. Acta neurol. scand. 45:513, 1969.

[13] 13. COHEN, S. & BANNIESER, R. - Immunoglobulin synthesis within the central nervous system in disseminated sclerosis. Lancet 1:366, 1967.

[14] 14. DELANK, H. W. & WREDE, M. T. - Der klinische Wart quantitativ-immunochemis-cher Bestimmungen verschiedener Proteine im Liquor cerebrospinalis. Klin. Wsch. 47:1270, 1969.

[15] 15. DENCKER, S. J. - Quantification of individual CSF proteins by immune precipitation in agar gel. J. Neurochem. 16:465, 1969.

[16] 16. DENCKER, S. J. - Quantification of individual proteins by immunodiffusion. Acta neurol. scand. 46 (suppl. 43):238, 1970.

[17] 17. EICKHOFF, K.; WIKSTROM, J.: POSER, S. & BAUER, H. - Protein profile of cerebrospinal fluid in multiple sclerosis with special reference to the function of the blood brain barrier. J. Neurol. 214:207, 1977.

[18] 18. FAHEY, J. L. & McKELVEY, E. M. - Quantitative determination of serum immunoglobulins in antibody agar plates. J. Immunol. 94:84, 1965.

[19] 19. FISCHER-WILLIAMS, M. & ROBERTS, R. C. - Cerebrospinal fluid proteins and serum immunoglobulins. Arch. Neurol. (Chicago) 25:526, 1971.

[20] 20. FOSSAN, G. O. - The transfer of IGG from serum to CSF evaluated by means of a naturally occurring antibody. Eur. Neurol. 15:231, 1977.

[21] 21. FOSSAN, G. O. & LARSEN, J. L. - Variation of IGG and total protein concentrations during lumbar cerebrospinal fluid collection. Acta neurol. scand. 57 (suppl. 67):278, 1978.

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