The effect of phenolated "vaccines" against experimental T. cruzi infection in mice

Menezes, Humberto

doi:10.1590/S0037-86821968000200003

Abstracts

"Vaccines" prepared from parasites of an avirulent cultivated Y strain of T. cruzi, suspended in phenolated 1/10.000 saline solution, with aluminum stearate, containing alive parasites, gave high degree of protection to mice against a posterior infection with virulent blood forms of the same parasites and strain. The degree of protection with 1/1000 and 1/10.000 phenol "vaccines", with no alive parasites, was very poor specially in the first group. The immunity seems to be related to the number of alive trypanosomes in the "vaccines".

Suspensões salinas fenoladas de Trypanosoma cruzi, cêpa Y, mantidas por 15 anos em meio de cultura artificial, desde que contenham alguns tripanosomas vivos, constituem bom meio de proteção, para camundongos, contra ulterior infecção com cêpa virulenta. O fenol a 1/10.000 determina alterações da forma e da motilidade dos parasitas o que faz supor uma redução maior ainda de sua capacidade infectante. O grau da imunidade parece relacionado com o número de parasitas vivos contidos nas "vacinas".

The effect of phenolated "vaccines" against experimental T. cruzi infection in mice

Humberto Menezes

Departamento de Patologia, Faculdade de Medicina de Ribeirão Prêto, S. Paulo, Brasil

ABSTRACT

"Vaccines" prepared from parasites of an avirulent cultivated Y strain of T. cruzi, suspended in phenolated 1/10.000 saline solution, with aluminum stearate, containing alive parasites, gave high degree of protection to mice against a posterior infection with virulent blood forms of the same parasites and strain.

The degree of protection with 1/1000 and 1/10.000 phenol "vaccines", with no alive parasites, was very poor specially in the first group.

The immunity seems to be related to the number of alive trypanosomes in the "vaccines".

RESUMO

Suspensões salinas fenoladas de Trypanosoma cruzi, cêpa Y, mantidas por 15 anos em meio de cultura artificial, desde que contenham alguns tripanosomas vivos, constituem bom meio de proteção, para camundongos, contra ulterior infecção com cêpa virulenta.

O fenol a 1/10.000 determina alterações da forma e da motilidade dos parasitas o que faz supor uma redução maior ainda de sua capacidade infectante.

O grau da imunidade parece relacionado com o número de parasitas vivos contidos nas "vacinas".

Full text available only in PDF format.

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This work has been supported by a Grant from Pfizer Química Ltda. Brasil.

Presented partially before the meeting of the Sociedade de Biologia, Ribeirão Prêto, September 21, 1967.

1 - BRENER, Z. - Contribuição ao estudo da terapêutica experimental da Doença de Chagas. Tese. Fac. Farm. Odont. Univ. Minas Gerais, Belo Horizonte, 1961.
2 - COLLIER, W. - Über Immunitát bei der Chagas Krankheit der Weissen maus. Z. Hyg. Infektionskr. 112: 88-92, 1931.
3 - EMMET, J. - Effect of X-radiation on Trypanosoma cruzi J. Parasit. 36: 45-47, 1950.
4 - FERNANDES, J. F.; HALSMAN, M. & CASTELLANI, O. - Effect of Actynomycin D on thp infectivity of Trypanosoma cruzi Nature 207; ... 1004-1005, 1965.
5 - JOHNSON, P. & NEAL, R.A. - Protective effect of killed Trypanosome vaccines with incorporated adjuvants. Nature: 200: 83, 1963.
6 - LAPIERRE, J. & ROUSSET, J. J. - Caractères biologiques d'une souche virulente de Trypanosome gambiense. Immunisation par vaccine tués. Bull. Soc. Path. Exot. 54: 336-345, 1961.
7 - LAVERAN, A. - Éssais d'immunisation contre des trypanosomes pathogens. Bull. Soc. Path. Exot. 5: .. 877-882, 1912.
8 - LAVERAN, A. & RONDSKY, D. - Éssais d'immunisation contre des trypanosomes pathogens. Trypanotoxines. Bull Soc. Path. Exot. 6: 176-181, 1913.
9 - MENEZES, H. - O emprêgo de adjuvantes na vacinação de camundongos com Trypanosoma cruzi II. Rev. Bras. Med. 22: 536-538, 1965.
10 - MENEZES, H. - The use of adjuvants in the vaccination of mice with lyophilized Trypanosoma cruzi I. O Hospital 68: 133-138, 1965.
11 - MENEZES, H. - Protective effect of an avirulent (cultivated) strain of Trypanosoma cruzi against experimental inféction in mice. Rev. Inst. Med. trop. S. Paulo 10: 1-4, 1968.
12 - MENEZES, H. - O emprêgo de "vacinas" na prevenção da tripanossomose cruzi experimental do camundongo. Communication to the Soc. Biologia. Ribeirão Prêto, Meeting of September 21, 1967.
13 - PIZZI, T. & PRAGER, R. - Immunidad a la sobreinfeccion inducida mediante cultivos de T. cruzi de virulência atenuada. Bol. Inf. Parasit. Chil. 7: 20-21, 1952.
14 - SANDERS A. & WALLACE, F.G. - Immunization of rats with irradiated T. lewisi Exp. Parasit. 18: 301-304 1966.
15 - SENECA, H. & PEER, P. - Immunobiological properties of chagastoxin (Lipopolysaccharide) . Trans. Roy. Soc. Trop. Med. & Hyg. 60: 610-620, 1966.
16 - SOLTYS, M.A. - Immunity in trypanosomiasis. V - Immunization of animais with dead Trypanosomes. Parasitology 54: 585-591, 1964.

Publication Dates

Publication in this collection
06 Aug 2013
Date of issue
Apr 1968

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

[1] 1 - BRENER, Z. - Contribuição ao estudo da terapêutica experimental da Doença de Chagas. Tese. Fac. Farm. Odont. Univ. Minas Gerais, Belo Horizonte, 1961.

[2] 2 - COLLIER, W. - Über Immunitát bei der Chagas Krankheit der Weissen maus. Z. Hyg. Infektionskr. 112: 88-92, 1931.

[3] 3 - EMMET, J. - Effect of X-radiation on Trypanosoma cruzi J. Parasit. 36: 45-47, 1950.

[4] 4 - FERNANDES, J. F.; HALSMAN, M. & CASTELLANI, O. - Effect of Actynomycin D on thp infectivity of Trypanosoma cruzi Nature 207; ... 1004-1005, 1965.

[5] 5 - JOHNSON, P. & NEAL, R.A. - Protective effect of killed Trypanosome vaccines with incorporated adjuvants. Nature: 200: 83, 1963.

[6] 6 - LAPIERRE, J. & ROUSSET, J. J. - Caractères biologiques d'une souche virulente de Trypanosome gambiense. Immunisation par vaccine tués. Bull. Soc. Path. Exot. 54: 336-345, 1961.

[7] 7 - LAVERAN, A. - Éssais d'immunisation contre des trypanosomes pathogens. Bull. Soc. Path. Exot. 5: .. 877-882, 1912.

[8] 8 - LAVERAN, A. & RONDSKY, D. - Éssais d'immunisation contre des trypanosomes pathogens. Trypanotoxines. Bull Soc. Path. Exot. 6: 176-181, 1913.

[9] 9 - MENEZES, H. - O emprêgo de adjuvantes na vacinação de camundongos com Trypanosoma cruzi II. Rev. Bras. Med. 22: 536-538, 1965.

[10] 10 - MENEZES, H. - The use of adjuvants in the vaccination of mice with lyophilized Trypanosoma cruzi I. O Hospital 68: 133-138, 1965.

[11] 11 - MENEZES, H. - Protective effect of an avirulent (cultivated) strain of Trypanosoma cruzi against experimental inféction in mice. Rev. Inst. Med. trop. S. Paulo 10: 1-4, 1968.

[12] 12 - MENEZES, H. - O emprêgo de "vacinas" na prevenção da tripanossomose cruzi experimental do camundongo. Communication to the Soc. Biologia. Ribeirão Prêto, Meeting of September 21, 1967.

[13] 13 - PIZZI, T. & PRAGER, R. - Immunidad a la sobreinfeccion inducida mediante cultivos de T. cruzi de virulência atenuada. Bol. Inf. Parasit. Chil. 7: 20-21, 1952.

[14] 14 - SANDERS A. & WALLACE, F.G. - Immunization of rats with irradiated T. lewisi Exp. Parasit. 18: 301-304 1966.

[15] 15 - SENECA, H. & PEER, P. - Immunobiological properties of chagastoxin (Lipopolysaccharide) . Trans. Roy. Soc. Trop. Med. & Hyg. 60: 610-620, 1966.

[16] 16 - SOLTYS, M.A. - Immunity in trypanosomiasis. V - Immunization of animais with dead Trypanosomes. Parasitology 54: 585-591, 1964.