Abstract
Many lines of Plasmodium falciparum undrgo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and selected cells produce gametocytes. The deletion also results in loss of expression of PfEMP1, the putative cytoadherence ligand, suggesting PfEMP1 or a regulatory gene controlling PfEMP1 expression and gametocytogenesis may be encoded in this region. We have isolated several markers for the deleted region and are currently using a YAC-P. falciparum library to investigate this region of the genome in detail.
chromosome deletion; cytoadherence; Plasmodium falciparum; pathology; PfEMP1
A chromosome 9 deletion in Plasmodium falciparum results in loss of cytoadherence
D. J. Kemp1
J. Thompson1
D. A. Barnes1
T. Triglia1
F. Karamalis1
C. Petersen2
G. V. Brown1
K. P. Day3
The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia
University of California, San Francisco, USA
Imperial College of Science and Technology, London, England
Many lines of Plasmodium falciparum undrgo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and selected cells produce gametocytes. The deletion also results in loss of expression of PfEMP1, the putative cytoadherence ligand, suggesting PfEMP1 or a regulatory gene controlling PfEMP1 expression and gametocytogenesis may be encoded in this region. We have isolated several markers for the deleted region and are currently using a YAC-P. falciparum library to investigate this region of the genome in detail.
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Publication Dates
-
Publication in this collection
04 June 2009 -
Date of issue
1992
