Czech J. Genet. Plant Breed., 2023, 59(4):189-195 | DOI: 10.17221/108/2022-CJGPB

The cytochrome P450 gene GhCYP94C1 is involved in drought stress in upland cotton (Gossypium hirsutum L.)Original Paper

Lijiao Gu1,2*, Pengyun Chen3, Shuxun Yu4*
1 Institute of Forest Biotechnology, Forestry College, Hebei Agricultural University, Baoding, P.R. China
2 Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, Baoding, P.R. China
3 Beijing Innovation Center for Crop Seed Technology, Ministry of Agriculture and Rural Affairs, Key Laboratory of Crop Heterosis Utilization, Ministry of Education, College of Agronomy and Biotechnology, China Agricultural University, Beijing, P.R. China
4 State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, P.R. China

Cytochrome P450 proteins belong to one of the largest families of enzyme proteins in plants and play important roles in plant growth and development and the stress response. In our previous studies, a cytochrome P450 gene, GhCYP94C1 (cytochrome P450 94C1), was functionally characterized as a positive regulator of seed germination, main root elongation and early flowering. However, whether the gene has other potential functions remains to be further explored. In our study, expression analysis showed that GhCYP94C1 was highly expressed in roots and was suppressed by drought treatment. Endogenous silencing of GhCYP94C1 via virus-induced gene silencing (VIGS) increased drought resistance in cotton plants, which was accompanied by the upregulated expression of the abscisic acid (ABA) biosynthesis gene nine-cis-epoxycarotenoid dioxygenase 9 (GhNCED9) during drought stress. Our findings suggested that GhCYP94C1 may play an important role in drought resistance. Combined with previous research results, the present results provide a theoretical basis for future breeding of new cotton varieties with early maturation and drought resistance.

Keywords: ABA signaling network; drought tolerance; expression analysis; qRT‒PCR; VIGS assay

Received: December 6, 2022; Accepted: February 21, 2023; Prepublished online: May 15, 2023; Published: September 11, 2023  Show citation

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Gu L, Chen P, Yu S. The cytochrome P450 gene GhCYP94C1 is involved in drought stress in upland cotton (Gossypium hirsutum L.). Czech J. Genet. Plant Breed.. 2023;59(4):189-195. doi: 10.17221/108/2022-CJGPB.
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