Hort. Sci. (Prague), 2008, 35(1):22-26 | DOI: 10.17221/648-HORTSCI

Micropropagation of Acacia chundra (Roxb.) DC.

G. R. Rout, S. K. Senapati, S. Aparajeta
Plant Biotechnology Division, Regional Plant Resource Centre, Orissa, India

An in vitro propagation of an economic leguminous tree, Acacia chundra, has been standardized. Induction of bud sprout was obtained from shoot tip and nodal explants derived from in vitro grown plants of A. chundra on the Murashige and Skoog (MS) basal medium supplemented with 6-benzylaminopurine (BA) (1.0 mg/l) and 20 mg/l adenine sulfate (Ads). The rate of multiplication was obtained on MS medium supplemented with 1.5 mg/l BA, 0.01 to 0.05 mg/l (indole-3-acetic acid) IAA and 50 mg/l Ads. The multiplication rate varied from 3 to 6 shoots depending on the growth regulators used. Excised shoots were rooted on half-strength MS basal salts supplemented with 0.25 mg/l indole-3-butyric acid (IBA) or IAA and 20 g/l (w/v) sucrose after 10 to 12 days of culture. The micropropagated plantlets have been acclimatized and successfully transferred to soil.

Keywords: growth regulators; in vitro; legume tree; micropropagation

Published: March 31, 2008  Show citation

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Rout GR, Senapati SK, Aparajeta S. Micropropagation of Acacia chundra (Roxb.) DC. Hort. Sci. (Prague). 2008;35(1):22-26. doi: 10.17221/648-HORTSCI.
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    References

    1. Anonymous, 1985. The wealth of India: A Dictionary of Indian Raw materials and Industrial Products. Vol. 1: A. New Delhi, Publications and Information Directorate, Council of Scientific and Industrial Research: 30.
    2. BECK S.L., DUNLOP R.W., 2001. Micropropagation of the Acacia species - A Review. In vitro Cellular and Developmental Biology - Plant, 37: 531-538. Go to original source...
    3. BECK S.L., DuNLOP R., Staden J.V., 2000. Meristem culture of Acacia mearnsii. Plant Growth Regulation, 32: 49-58. Go to original source...
    4. DEWAN A., NANDA K., GUPTA S.C., 1992. In vitro micropropagation of Acacia nilotica subsp. Indica Brenan via cotyledonary nodes. Plant Cell Reports, 12: 18-21. Go to original source... Go to PubMed...
    5. Dhar U., Upreti J., 1999. In vitro regeneration of a mature leguminous liana (Bauhinia vahlii Wight and Arnott.). Plant Cell Reports, 18: 664-669. Go to original source...
    6. Harter H.L., 1960. Critical values for Duncan's multiple range test. Biometric, 16: 671-685. Go to original source...
    7. Lakshmi Sita G., Sreenatha K.S., SUJATA S., 1992. Plantlet production from shoot tip cultures of red sandalwood (Pterocarpus santalinus L.). Current Science, 62: 532-535.
    8. Mittal A., Agarwal R., Gupta S.C., 1989. In vitro development of plantlets from axillary buds of Acacia auriculiformis - a leguminous tree. Plant Cell, Tissue and Organ Culture, 19: 65-70. Go to original source...
    9. Monteuuis O., Bon M.C., 2000. Influence of auxins and darkness on in vitro rooting of micropropagated shoots from mature and juvenile Acacia mangium. Plant Cell, Tissue and Organ Culture, 63: 173-177. Go to original source...
    10. Murashige T., Skoog F., 1962. A revised media for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497. Go to original source...
    11. Nanda R.M., Das P., ROUT G.R., 2004. In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker. Annals of Forest Science, 61: 381-386. Go to original source...
    12. Raghava Swamy B.V., Himabindu K., Lakshmi Sita G., 1992. In vitro micropropagation of elite rosewood (Dalbergia latifolia Roxb.). Plant Cell Reports, 11: 126-131. Go to original source... Go to PubMed...
    13. Skolmen R.G., 1986. Acacia (Acacia koa Gray.). In: Bajaj Y.P.S. (ed.), Biotechnology in Agriculture and Forestry, Trees - 1. Berlin, Springer-Verlag: 375-384.
    14. Tomar U.K., Gupta S.C., 1988. In vitro plant regeneration of leguminous trees (Albizia spp.). Plant Cell Reports, 7: 385-388. Go to original source... Go to PubMed...
    15. Upreti J., Dhar U., 1996. Micropropagation of Bauhinia vahlii Wight & Arnott. - a leguminous liana. Plant Cell Reports, 16: 250-254. Go to original source...
    16. Hort. Sci. (Prague), 35, 2008 (1): 22-26 BAZHAGAN V.R., 2002. In vitro propagation of Acacia sinuata (Lour.) Merr. Via. cotyledonary nodes. Agroforestry Systems, 55: 9-15. Go to original source...
    17. VENGADESAN G., GANAPATHI A., ANAND R.P., SELVARAJ N., 2003. In vitro propagation of Acacia sinuata (Lour.) Merr. from nodal segments of a 10-year-old tree. In Vitro Cellular and Developmental Biology - Plant, 39: 409-414. cropropagation of Morus nigra L. from shoot tip and nodal explants of mature trees. Scientia Horticulturae, 44: 61-67. Go to original source...

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