Vet Med - Czech, 2001, 46(5):140-144 | DOI: 10.17221/7870-VETMED

Protective role of supplemental vitamin E on lipid peroxidation, vitamins E, A and some mineral concentrations of broilers reared under heat stressOriginal Paper

K. Sahin, N. Sahin, M. Onderci, S. Yaralioglu, O. Kucuk
1 Department of Animal Nutrition, Veterinary Faculty, University of Firat, Elazig, Turkey
2 Veterinary Control and Research Institute, Elazig, Turkey
3 Department of Biochemistry, Veterinary Faculty, University of Firat, Elazig, Turkey

An experiment utilizing Cobb-500 male broilers was conducted to evaluate the effects of vitamin E supplementation at various concentrations on malonyldialdehyde (MDA) as an indicator of lipid peroxidation, serum and liver concentrations of antioxidant vitamins and some minerals of broilers reared under heat stress (32°C). One day-old 150 male broilers were randomly assigned to 5 treatment groups, 3 replicates of 10 birds each. The birds received either a basal diet or basal diet supplemented with vitamin E (dl-a-tocopherol acetate) at 62.5, 125, 250, or 500 mg/kg of diet. Increased supplemental vitamin E linearly increased serum vitamin E and A, but decreased (P = 0.001) MDA concentrations. Increasing dietary vitamin E supplementation also resulted in linear increases in liver vitamin E and A concentrations, but linear decreases in MDA concentrations (P = 0.01). Increasing dietary vitamin E caused a linear increase in serum concentrations of Fe and Zn (P= 0.001), but a decrease in serum concentration of Cu (P = 0.001). Results of the present study conclude that in broiler chicks reared under heat stress a 250 mg of vitamin E supplementation can be considered as a protective management practice in a broiler diet, reducing the negative effects of heat stress.

Keywords: malonyldialdehyde; vitamin A; vitamin E; Fe, Zn, Cu

Published: May 31, 2001  Show citation

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Sahin K, Sahin N, Onderci M, Yaralioglu S, Kucuk O. Protective role of supplemental vitamin E on lipid peroxidation, vitamins E, A and some mineral concentrations of broilers reared under heat stress. Vet Med-Czech. 2001;46(5):140-144. doi: 10.17221/7870-VETMED.
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References

  1. A.O.A.C. (1990): Official Methods of Analysis Association of Agricultural Chemists Virginia. D. C., U.S.A.
  2. Beisel W.R. (1982): Single nutrients and immunity. Am. J. Clin. Nutr., 35, 442–451. Go to original source... Go to PubMed...
  3. Bollengier-Lee S., Mitchell M.A., Utomo D.B., Williams P.E.V., Whitehead C.C. (1998): Influence of high dietary vitamin E supplementation on egg production and plasma characteristics in hens subjected to heat stress. Brit. Poultry Sci., 39, 106–112. Go to original source... Go to PubMed...
  4. Bollengier-Lee S., Williams P.E.V., Whitehead C.C. (1999): Optimal dietary concentration of vitamin E for alleviating the effect of heat stress on egg production in laying hens. Brit. Poultry Sci., 40, 102–107. Go to original source... Go to PubMed...
  5. Brubacher G., Scharer K., Studer A., Wiss O. (1964): Uber die gegensetiege Beeinflussung von vitamin E, vitamin A and carotinoiden. Z. Ernahrungswiss., 5, 190–202. Go to original source... Go to PubMed...
  6. Burton G., Ingold K.U. (1984): β-Carotene: An unusual type of lipid antioxidant. Science, 224, 569–573. Go to original source... Go to PubMed...
  7. Butler E.J., Curtis M.J. (1973): The effect of Escherichia coli endotoxin and ACTH on the plasma zinc concentration of the domestic fowl. Res. Vet. Sci., 15, 367–269. Go to original source...
  8. Donkoh A. (1989): Ambient temperature: a factor affecting performance and physiological response of broiler chickens. Int. J. Biometeorol., 33, 259–265. Go to original source... Go to PubMed...
  9. Feenster R. (1985): High temperatures decrease vitamin utilization. Mis. Poult., 38–41.
  10. Frank A., Pehrson B., Petersson L.R. (1986): Concentration of some important elements in liver of young cattle supplemented with selenite enriched feed. J. Vet. Med. A., 33, 4212–4225. Go to original source... Go to PubMed...
  11. Gallo-Torres D.C. (1980): Absorption, blood transport and metabolism of vitamin E. In: Maclin L.J. (ed.): A Comprehensive Treatise. Marcel Dekker, New York. 170–267.
  12. Halliwell B., Gutteridge J.M.C. (1989): Free Radicals in Biology and Medicine. 2nd ed. Oxford University Press, New York.
  13. Hurwitz S., Weiselberg M., Eisner U., Bartov I., Riesenfeld G., Sharvit M., Niv A., Bornstein S. (1980): The energy requirements and performance of growing chickens and turkeys, as affected by environmental temperature. Poultry Sci., 59, 2290–2299. Go to original source...
  14. Klasing K.C. (1998): Comparative Avian Nutrition. University Press, Cambridge. 277–299. Go to original source...
  15. Kornburst D. J., Mavis R.D. (1980): Relative susceptibility of microsomes from lung, heart, liver, kidney, brain and testes to lipid peroxidation: Correlation with vitamin E content. Lipids, 15, 315–320. Go to original source... Go to PubMed...
  16. Kutsky R J. (1981): Handbook of Vitamins, Minerals and Hormones. 2nd ed. VNR, New York. 157–207.
  17. Linder M.C. (1991): Nutrition and metabolism of the trace elements. In: Linder M.C. (ed.): Nutritional Biochemistry and Metabolism with Clinical Applications. Elsevier, New York. 215–276.
  18. Matkovics B., Szabo I., Varga I.S. (1989): Determination of enzyme activities in lipid peroxidation and glutathione pathways. Lab. Diagn., 15, 248–249.
  19. McDowell L.R. (1989): Vitamins in Animal Nutrition – Comparative Aspects to Human Nutrition. Vitamin A and E. Academic Press, London. 10–52, 93–131. Go to original source...
  20. McMurry C.H., Blanchflower W.J., Rice D.A. (1980): Influence of extraction techniques on the determination of α-tocopherol in animal feedstuffs, J. Assoc. Off. Anal. Chem., 63, 1258–1261. Go to original source... Go to PubMed...
  21. Morrissey P.A., Brandon S., Buckley D.J., Sheehy P.J.A., Frigg M. (1997): Tissue content of α-tocopherol and oxidative stability of broilers receiving dietary α-tocoperyl acetate supplement for various periods post-slaughter. Brit. Poultry Sci., 38, 84–88. Go to original source... Go to PubMed...
  22. National Research Council (1994): Nutrient requirements of poultry National Academy Press. Washington, D.C. Ninth Revised Edition.
  23. Naziroðlu M., ªahin K., ªimºek H., Aydilek N., Ertaº O.N. (2000): The effect of food withdrawal and darkening on lipid peroxidation of laying hens in high ambient temperatures. Dtsch. Tierarztl. Wschr., 107, 199–202.
  24. Packer L. (1991): Protective role of vitamin E in biological systems. Am. J. Clin. Nutr., 53, 1050–1055. Go to original source... Go to PubMed...
  25. Packer L., Landvik S. (1990): Vitamin E in biological systems. In: Emerit I., Packer L., Auclair (eds.): Antioxidants in Therapy and Preventive Medicine. Plenum, New York. 93–103. Go to original source...
  26. Placer Z.A., Cushmann L.L., Johnson B.C. (1966): Estimation of products of lipid peroxidation in biochemical systems. Anal. Biochem., 16, 359–364. Go to original source... Go to PubMed...
  27. Pond W.G., Church D.C., Pond K.R. (1995): Zinc. In: Basic Animal Nutrition and Feeding. John Wiley & Sons. Fourth Ed. New York. 190–193.
  28. Putnam M.E., Comben N. (1987): Vitamin E. Vet. Rec., 121, 541–545. Go to PubMed...
  29. SAS Institute (1989): SAS® User’s Guide: Statistics. SAS Institute Inc., Cary, NC.
  30. Sunder A., Richter G., Flachowsky G. (1997): Influence of different concentrations of vitamin E in the feed of laying hens on the vitamin E-transfer into the egg. Proc. Soc. Nutr. Physiol., 6, 14–152.
  31. Sahin K., Ertaº O.N., Güler T. (1999): Sicaklik stresi altindaki yumurta tavuklarinda farkli yemleme yöntemlerinin vitamin A, vitamin E ile bazi kan parametreleri üzerine etkileri. In: Serbest Radikaller ve Antioksidanlar Araºtirma Dernegi II, Ulusal Kongresi. P-24 58.
  32. Tufft L.S., Nockles C.F. (1991): The effects of stress, Escherichia coli, dietary Ethylenediamintetraacetic acid, and their interaction on tissue trace elements in chicks. Poultry Sci., 70, 2439–2449. Go to original source... Go to PubMed...
  33. Van Saun R.J. (1990): Rational approach to selenium supplementation essential. Feedstuffs, 15, 15–18.
  34. Whitehead C.C., Bollengier-Lee S., Mitchell M.A., Williams P.E.V. (1998): Vitamin E can alleviate the depression in egg production in heat stressed laying hens. In: Proc. of Spring meeting, WPSA-UK Branch. 55–56. Scarborough.

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