INCORPORATION OF RADIOACTIVE PRECURSORS INTO THE ALKALOIDS OF ORGANIZED CULTURES FROM LOBELIA INFLATA AND THEIR VEGETATIVE MICROPROPAGATION

Continuing our research we studied the biomass and alkaloid production of in vitro and in vivo organized cultures from Lobelia inflata.

On the base of qualitative and quantitative chromatographic results - isolation and spectroscopic (¹H-NMR, ¹³C-NMR, MS) identification of the major compounds as well as comparison with intact plant and authentic substances- it was stated that the organized cultures have the ability to synthetize the characteristic alkaloids of intact plant. Special attention was taken to the study of lobeline content because of its important role in medicine. The total alkaloid and lobeline contents were determined by photometric and densitometric methods respectively.

The new cytokinin-like regulators (N, -n-isopropyl-benzimidasolium-chloride and dihydroxy-phtalazine-diguanidine--chloride) had more positive influence on the biomass and alkaloid content in the organized Lobelia inflata than kinetin.

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