ISHS Acta Horticulturae 1000: VII International Symposium on New Floricultural Crops
PROPAGATION SYSTEMS FOR LOBELIA BRIDGESII, AN ENDANGERED SPECIES FROM SOUTHERN CHILE WITH ORNAMENTAL POTENTIAL |
| Authors: | P. Seemann , J. Carrasco |
| Keywords: | acclimation, micropropagation, root induction, rooting, germination |
| DOI: | 10.17660/ActaHortic.2013.1000.36 |
| Abstract:
Lobelia bridgesii Hook. et Arn. is a native species to Chile (39° to 40°S lat.) commonly called pink tupa or devil’s tobacco. In culture, it is possible to be grown in sunny areas on rocky and well drained soils. It is reasonably hardy, but requires protection from winter wetness and frost. Propagation studies were carried out which included germination experiments under either stratification or GA3 treatments, achieving a mean germination of 38 to 59.3%. Cutting propagation in early or mid-summer and early fall, led to poor rooting responses (max. 33% with 500 ppm IBA), using herbaceous cuttings. Starting from young vegetative propagated plants, micropropagation experiments were conducted using different basal media, supplemented with NAA (0-0.2 mg/L) and/or BAP (0-2 mg/L) under controlled growth conditions, which led to good survival rates (up to 100%). Media with 0.1 mg/L NAA+1.0 or 2.0 mg/L BAP gave best shoot development. When transferred to different rooting media, supplemented with up to 0.75 mg/L NAA, plants showed good rooting responses (60-100%) and up to 11 roots/explant. Further experiments on acclimation to ex vitro conditions led to 73.3-100% survival rates and good leaf development, indicating that tissue culture is a suitable propagation system to start commercial propagation. | |
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