초록

Mechanical stimulation has been reported to improve cell growth in bone and cartilage regeneration. Various research groups have described their own bioreactors that stimulate cell-seeded scaffolds. We have developed a bioreactor capable of applying controlled compressive loading to a cell-encapsulated agarose hydrogel. To compare the effect of mechanical stimulation on agarose hydrogel, we used one control group (static mode) and five experimental groups. The results show that the intermittent_3sec conditions improve proliferation of cells better than the other conditions studied.

키워드

Mechanical stimulation(기계적 자극), Hydrogel scaffold(하이브리드 인공지지체), Agarose(아가로즈), Tissue engineering(조직공학), Bioreactor(동적배양장치).

참고문헌(13)open

  1. [학술지] Robert L. Mauck / 2000 / Functional Tissue Engineering of Articular Cartilage Through Dynamic Loading of Chondrocyte-Seeded Agarose Gels / Journal of Biochemical Engineering 122 (3) : 252 ~ 260

  2. [학술지] John D. Kisiday / 2004 / Effects of dynamic compressive loading on chondrocyte biosynthesis in self-assembling peptide scaffold / Journal of Biome-chanics 37 (5) : 594 ~ 604

  3. [학술지] U. Meyer / 2006 / Design and performance of a bioreactor system for mechanically promoted threedimensional tissue engineering / British Journal of Oral and Maxillofacial Surgery 44 (2) : 134 ~ 140

  4. [학술지] Z. Chunqiu / 2006 / Direct compression as an appropriately mechanical environment in bone tissue reconstruction in vitro / Med. Hypothese 67 (6) : 1414 ~ 1418

  5. [학술지] Hunter, C. J. / 2004 / Dynamic compression of chondrocyte-seeded fibrin gels : effects on matrix accumulation and mechanical stiffness / Osteo Arth-ritis and Cartilage 12 (2) : 117 ~ 130

  6. [학술지] Orahn Preiss-Bloom / 2009 / Real-time monitoring of force response measured in mechanically stimulated tissue-engineered cartilage / Artificial Organs 33 (4) : 318 ~ 327

  7. [학술지] Taly P. Appelman / 2009 / The differential effect of scaffold composition and architecture on chondrocyte response to mechanical stimulation / Biomaterials 30 (4) : 518 ~ 525

  8. [학술지] G. E. Nugent-Derfus / 2007 / Continuous passive motion applied to whole joints stimulates chondrocyte biosynthesis of PRG4 / Osteo Arthriti-sand Cartilage 15 (5) : 556 ~ 574

  9. [학술지] S. -J. Lee / 2009 / Development of a hybrid scaffold and a bioreactor for cartilage regeneration / Chinese Sci. Bulletin 54 (19) : 3608 ~ 3612

  10. [학술지] Awad, H. A. / 2004 / Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate and gelatin scaffolds / Biomaterials 25 (16) : 3211 ~ 3222

  11. [학술지] H. Towbin / 1979 / Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : procedure and some applications / Proc. Natl. Acad. Science 76 (9) : 4350 ~ 4354

  12. [학술지] 이승재 / 2012 / Performance Analysis of Scaffold Fabrication System Based on Micro-stereolithography Technology / 한국기계기술학회지 14 (6) : 11 ~ 16

  13. [학술지] 이승재 / 2011 / 광섬유를 이용한 미세 광 기계식 가속도 센서의 개발 / 한국기계기술학회지 13 (4) : 93 ~ 100