Effect of cytokinin and cultivar on shoot formation of Gerbera jamesonii in vitro.

Abstract

The success of gerbera clonal propagation in vitro, using either capitulum explants or subcultured shoots in trials with up to 28 cvs, depended both on the cv. and on the cytokinin level in the medium. With capitulum explants, shoot formation was very low for some cvs regardless of the level of BA (5, 10 or 20 mg/l) whereas other cvs had individual optimum BA levels. Axillary branching of subcultured shoots differed between cvs and between levels of kinetin (1, 5 or 10 mg/l) in the medium. The optimum level for the highest quality shoots (highest leaf weight/shoot, no leaf malformation and no callus formation) was not always the same as the level producing the highest number of axillary shoots. Results are tabulated for each cv. (Abstract retrieved from CAB Abstracts by CABI’s permission)