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Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

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Aquaculture Fish Diseases Fish Health Management

Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb.) on the Immune Response and Gene Expression of Nile Tilapia (Oreochromis niloticus) Infected with Aeromonas hydrophila

https://doi.org/10.20473/jipk.v16i1.44079
Arini Resti Fauzi
Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia
https://orcid.org/0000-0002-0162-6895
Munti Yuhana
Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia
https://orcid.org/0000-0002-4019-0721
Widanarni Widanarni
Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia
https://orcid.org/0000-0002-0821-3225
Mia Setiawati
Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia
https://orcid.org/0000-0002-6604-8184
Usamah Afiff
Department of Veterinary Disease and Public Health, Veterinary and Biomedical School, IPB University, Bogor, West Java. Indonesia
https://orcid.org/0000-0002-1373-9520

Corresponding Author(s) : Munti Yuhana

muntiyu@apps.ipb.ac.id

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract


Abstract


Dayak onion (Eleutherine bulbosa (Mill.) Urb.) has the potential to be an immunostimulant to benefit fish health. This study aimed to evaluate the effectiveness of dietary supplementation with the powder and crude ethanol extracts of Dayak onion on the hematology and immune response parameters of Nile tilapia challenged with Aeromonas hydrophila. The research used a completely randomized design with six treatments and three replications. Nile tilapias were fed with Dayak onion (w/v) consisting of powders of 5% (P5), 10% (P10), and 15% (P15), while crude extract was 0.5% (E05) and given during rearing for 30 days. Positive control (C+) and negative control (C-) were used without Dayak onion. Nile tilapia in treatment C+, P5, P10, and P15 were injected with A. hydrophila 10⁶ CFU mL⁻1, while that in treatment C- was injected with phosphate buffered saline (PBS) intramuscularly. The results showed that the dietary with 15% powder and 0.5% crude extract significantly improved the hematology and immune response parameters compared to the control after the challenge against A. hydrophila (P<0.05). Dayak onion supplementation effectively increased the health status based on hematology and immune response parameters of Nile tilapia against A. hydrophila infection.


Highlight Research



  1. Dayak onion has significant potential to improve nonspecific immunity in fish.

  2. Dayak onion improves hematology and immune response of Nile tilapia.

  3. Dayak onion can significantly regulate the immune system against A. hydrophila infection.

  4. Dayak onion significantly increases the production of the gene expression of IL-1β and TNF-α.

Keywords

Eleutherine bulbosa gene expression Histopathology Immunostimulant
Fauzi, A. R., Yuhana, M., Widanarni, W., Setiawati, M., & Afiff, U. (2023). Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb.) on the Immune Response and Gene Expression of Nile Tilapia (Oreochromis niloticus) Infected with Aeromonas hydrophila. Jurnal Ilmiah Perikanan Dan Kelautan, 16(1), 1–14. https://doi.org/10.20473/jipk.v16i1.44079
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References
  1. Aluta, U. P., Aderolu, A. Z., Lawal, M. O., & Olutola, A. A. (2021). Inclusion effect of onion peel powder in the diet of African catfish, Clarias gariepinus: Growth, blood chemistry, hepatic antioxidant enzymes activities and SOD mRNA responses. Scientific African, 12:e00780.
  2. Amenyogbe, E., Yang, E., Xie, R., Huang, J., & Chen, G. (2022). Influences of indigenous isolates Pantoea agglomerans RCS2 on growth, proximate analysis, haematological parameters, digestive enzyme activities, serum biochemical parameters, antioxidants activities, intestinal morphology, disease resistance, and molecul. Aquaculture, 551(11):737942.
  3. Anderson, D. P., & Siwicki, A. K. (1995). Basic hematology and serology for fish health programs. Manila: Fish Health Section, Asian Fisheries Society.
  4. Blaxhall, P. C., & Daisley, K. W. (1973). Routine haematological methods for use with fish blood. Journal of Fish Biology, 5(6):771–781.
  5. Chen, Y. Bin, Hu, J., Lyu, Q. J., Liu, L. J., Wen, L. F., Yang, X. K., & Zhao, H. H. (2016). The effects of Natucin C-Natucin P mixture on blood biochemical parameters, antioxidant activity and non-specific immune responses in tilapia (Oreochromis niloticus). Fish & Shellfish Immunology, 55:367–373.
  6. Chen, N., Jiang, J., Gao, X., Li, X., Zhang, Y., Liu, X., Yang, H., Bing, X., & Zhang, X. (2018). Histopathological analysis and the immune related gene expression profiles of mandarin fish (Siniperca chuatsi) infected with Aeromonas hydrophila. Fish & Shellfish Immunology, 83:410–415.
  7. Dawood, M. A. O., Abdo, S. E., Gewaily, M. S., Moustafa, E. M., SaadAllah, M. S., AbdEl-kader, M. F., Hamouda, A. H., Omar, A. A., & Alwakeel, R. A. (2020). The influence of dietary β-glucan on immune, transcriptomic, inflammatory and histopathology disorders caused by deltamethrin toxicity in Nile tilapia (Oreochromis niloticus). Fish & Shellfish Immunology, 98(11):301–311.
  8. Derome, N., Gauthier, J., Boutin, S., & Llewellyn, M. (2016). Bacterial opportunistic pathogens of fish. In C. J. Hurst (Ed.), The Rasputin effect: When commensals and symbionts become parasitic. (pp. 81–108). Switzerland: Springer Cham.
  9. Dotta, G., de Andrade, J. I. A., Garcia, P., Alves Jesus, G. F., Mouriño, J. L. P., Mattos, J. J., Dias Bainy, A. C., & Martins, M. L. (2018). Antioxidant enzymes, hematology, and histology of spleen in Nile tilapia fed supplemented diet with natural extracts challenged with Aeromonas hydrophila. Fish & Shellfish Immunology, 79:175–180.
  10. Elala, N. M. A., & Ragaa, N. M. (2015). Eubiotic effect of a dietary acidifier (Potassium diformate) on the health status of cultured Oreochromis niloticus. Journal of Advanced Research, 6(4):621–629.
  11. El-Kady, A. A., Magouz, F. I., Mahmoud, S. A., & Abdel-Rahim, M. M. (2022). The effects of some commercial probiotics as water additive on water quality, fish performance, blood biochemical parameters, expression of growth and immune-related genes, and histology of Nile tilapia (Oreochromis niloticus). Aquaculture, 546:737249.
  12. Fazio, F. (2019). Fish hematology analysis as an important tool of aquaculture: A review. Aquaculture, 500:237–242.
  13. Gauthier, D. T. (2015). Bacterial zoonoses of fishes: A review and appraisal of evidence for linkages between fish and human infections. The Veterinary Journal, 203(1):27–35.
  14. Gomes, A. R. Q., da Rocha Galucio, N. C., de Albuquerque, K. C. O., Brígido, H. P. C., Varela, E. L. P., Castro, A. L. G., Vale, V. V., Bahia, M. O., Rodriguez Burbano, R. M., de Molfeta, F. A., Carneiro, L. A., Percario, S., & Dolabela, M. F. (2021). Toxicity evaluation of Eleutherine plicata Herb. extracts and possible cell death mechanism. Toxicology Reports, 8:1480–1487.
  15. Hadfield, G. (2009). Atlas of histology. British Medical Journal, 1:748.
  16. Haerani, A., Chaerunisa, A. Y., & Subarnas, A. (2019). Antioxidant Activities of Muntingia calabura, Syzygium cumini, Ocimum basilicum, and Eleutherine bulbosa using DPPH Method. Indonesian Journal of Pharmaceutics, 1(2):57–61.
  17. Harikrishnan, R., Balasundaram, C., & Heo, M. S. (2010). Herbal supplementation diets on hematology and innate immunity in goldfish against Aeromonas hydrophila. Fish & Shellfish Immunology, 28(2):354–361.
  18. Harikrishnan, R., Devi, G., Doan, H. Van, Tapingkae, W., Balasundaram, C., Arockiaraj, J., & Ringø, E. (2022). Changes in immune genes expression, immune response, digestive enzymes -antioxidant status, and growth of catla (Catla catla) fed with Astragalus polysaccharides against edwardsiellosis disease. Fish & Shellfish Immunology, 121:418–436.
  19. Harikrishnan, R., Naafar, A., Musthafa, M. S., Ahamed, A., Arif, I. A., & Balasundaram, C. (2018). Effect of Agaricus bisporus enriched diet on growth, hematology, and immune protection in Clarias gariepinus against Flavobacterium columnare. Fish & Shellfish Immunology, 73:245–251.
  20. Insanu, M., Kusmardiyani, S., & Hartati, R. (2014). Recent studies on phytochemicals and pharmacological effects of Eleutherine Americana Merr. Procedia Chemistry, 13:221–228.
  21. Izwar, A., Nuryati, S., Sukenda, Rahman, & Purnomowati, R. (2020). Isolation, identification, and pathogenicity tests of pathogenic bacterial associated with black body syndrome in white barramundi Lates calcarifer B. Jurnal Akuakultur Indonesia, 19(1):39–49.
  22. Kuswoyo, T., Isnansetyo, A., Murwantoko, Husni, A., & Istiqomah, I. (2023). Sodium alginate from Padina australis modulates innate immune and immune gene expression in red tilapia (Oreochromis sp.). Jurnal Ilmiah Perikanan dan Kelautan, 15(1):1–14.
  23. Liew, F. Y. (2003). The role of innate cytokines in inflammatory response. Immunology Letters, 85(2):131–134.
  24. Liu, X., Deng, H., Xu, Q., Luo, K., Zhou, J., Gao, W., Wang, Z., Zhang, H., & Zhou, X. (2022). Effects of tea tree essential oil supplementation in low fish meal diet on growth, lipid metabolism, anti-oxidant capacity and immunity of largemouth bass (Micropterus salmoides). Aquaculture Reports, 27:101387.
  25. Liu, C., Liu, H., Han, D., Xie, S., Jin, J., Yang, Y., & Zhu, X. (2020). Effects of dietary Arthrospira platensis supplementation on the growth performance, antioxidation and immune related-gene expression in yellow catfish (Pelteobagrus fulvidraco). Aquaculture Reports, 17:100297.
  26. Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods, 25(4):402–408.
  27. MacKenzie, S., Planas, J. V., & Goetz, F. W. (2003). LPS-stimulated expression of a tumor necrosis factor-alpha mRNA in primary trout monocytes and in vitro differentiated macrophages. Developmental & Comparative Immunology, 27(5):393-400.
  28. Mehrabi, Z., Firouzbakhsh, F., Rahimi-Mianji, G., & Paknejad, H. (2020). Immunity and growth improvement of rainbow trout (Oncorhynchus mykiss) fed dietary nettle (Urtica dioica) against experimental challenge with Saprolegnia parasitica. Fish & Shellfish Immunology, 104:74–82.
  29. Mohammadi, G., Rashidian, G., Hoseinifar, S. H., Naserabad, S. S., & Doan, H. Van. (2020). Ginger (Zingiber officinale) extract affects growth performance, body composition, haematology, serum and mucosal immune parameters in common carp (Cyprinus carpio). Fish & Shellfish Immunology, 99:267–273.
  30. Mohammadian, T., Alishahi, M., Tabandeh, M. R., Jangaran Nejad, A., Karami, E., & Zarea, M. (2019). Effects of autochthonous probiotics, isolated from Tor grypus (Karaman, 1971) intestine and Lactobacillus casei (PTCC 1608) on expression of immune-related genes. Aquaculture International, 27(1):239–260.
  31. Moustafa, E. M., Dawood, M. A. O., Assar, D. H., Omar, A. A., Elbialy, Z. I., Farrag, F. A., Shukry, M., & Zayed, M. M. (2020). Modulatory effects of fenugreek seeds powder on the histopathology, oxidative status, and immune related gene expression in Nile tilapia (Oreochromis niloticus) infected with Aeromonas hydrophila. Aquaculture, 515:734589.
  32. Munaeni, W., Pariakan, A., Yuhana, M., Setiawati, M., & Abidin, L. O. B. (2017). In vitro phytochemical and inhibitory potential test of bawang hutan bulb extract (Eleutherine palmifolia) on Vibrio harveyi. Microbiology Indonesia, 11(3):75–80.
  33. Munaeni, W., Widanarni, W., Yuhana, M., Setiawati, M., & Wahyudi, A. T. (2019). Phytochemical analysis and antibacterial activities of Eleutherine bulbosa (Mill.) Urb. extract against Vibrio parahaemolyticus. Asian Pacific Journal of Tropical Biomedicine, 9(9):397-404.
  34. Munaeni, W., Widanarni, W., Yuhana, M., Setiawati, M., & Wahyudi, A. T. (2020a). The potential of buton forest onion Eleutherine bulbosa (Mill.) Urb. extract as a prebiotic and an antioxidant. Journal of Microbiology, Biotechnology and Food Sciences, 10(1):107–111.
  35. Munaeni, W., Widanarni, Yuhana, M., Setiawati, M., & Wahyudi, A. T. (2020b). Impact of dietary supplementation with Eleutherine bulbosa (Mill.) Urb. on intestinal microbiota diversity and growth of white shrimp, Litopenaeus vannamei. Aquaculture, 528(5):735466.
  36. Nayak, S., Yogev, U., Kpordzaxor, Y., Zhu, Z., Gur, N., Gross, A., & Zilberg, D. (2023). From fish excretions to high-protein dietary ingredient: Feeding intensively cultured barramundi (Lates calcarifer) a diet containing microbial biomass (biofloc) from effluent of an aquaculture system. Aquaculture, 562:738780.
  37. Novaryatiin, S., Ramli, A., & Ardhany, S. D. (2019). Uji daya hambat ekstrak etanol bawang dayak (Eleutherine bulbosa (Mill.) Urb.) terhadap bakteri Staphylococcus aureus. Jurnal Surya Medika, 4(2):51–59.
  38. Padhi, L., & Panda, S. K. (2015). Antibacterial activity of Eleutherine bulbosa against multidrug-resistant bacteria. Journal of Acute Medicine, 5(3):53–61.
  39. Ren, Z., Wang, S., Cai, Y., Wu, Y., Tian, L., Liao, J., Wang, S., Jiang, L., Guo, W., & Zhou, Y. (2020). Antioxidant capacity, non-specific immunity, histopathological analysis and immune-related genes expression in Nile tilapia Oreochromis niloticus infected with Aeromonas schubertii. Aquaculture, 529(5):735642.
  40. Rodríguez, A., Esteban, M. A., & Meseguer, J. (2003). Phagocytosis and peroxidase release by seabream (Sparus aurata L.) leucocytes in response to yeast cells. The Anatomical Record, 272A(1):415–423.
  41. Sherif, A. H., Eldessouki, E. A., Sabry, N. M., & Ali, N. G. (2022). The protective role of iodine and MS-222 against stress response and bacterial infections during Nile tilapia (Oreochromis niloticus) transportation. Aquaculture International, 31:401–416.
  42. Shi, P., Du, W., Wang, Y., Teng, X., Chen, X., & Ye, L. (2019). Total phenolic, flavonoid content, and antioxidant activity of bulbs, leaves, and flowers made from Eleutherine bulbosa (Mill.) Urb. Food Science and Nutrition, 7(1):148–154.
  43. Sutthi, N., Panase, A., Chitmanat, C., Sookying, S., Ratworawong, K., & Panase, P. (2020). Effects of dietary leaf ethanolic extract of Apium graveolens L. on growth performance, serum biochemical indices, bacterial resistance and lysozyme activity in Labeo chrysophekadion (Bleeker, 1849). Aquaculture Reports, 18:100551.
  44. Tattiyapong, P., Dachavichitlead, W., & Surachetpong, W. (2017). Experimental infection of Tilapia Lake Virus (TiLV) in Nile tilapia (Oreochromis niloticus) and red tilapia (Oreochromis spp.). Veterinary Microbiology, 207:170–177.
  45. Telli, G. S., Ranzani-Paiva, M. J. T., Dias, D. de C., Sussel, F. R., Ishikawa, C. M., & Tachibana, L. (2014). Dietary administration of Bacillus subtilis on hematology and non-specific immunity of Nile tilapia Oreochromis niloticus raised at different stocking densities. Fish & Shellfish Immunology, 39(2):305–311.
  46. Wedemeyer, G. A., & Yasutake, W. T. (1977). Clinical methods for the assessment of the effects of environmental stress on fish health (Vol. 89). Washington D.C.: Fish and Wildlife Service.
  47. Wolf, J. C., Baumgartner, W. A., Blazer, V. S., Camus, A. C., Engelhardt, J. A., Fournie, J. W., Frasca, S., Groman, D. B., Kent, M. L., Khoo, L. H., Law, J. M., Lombardini, E. D., Ruehl-Fehlert, C., Segner, H. E., Smith, S. A., Spitsbergen, J. M., Weber, K., & Wolfe, M. J. (2015). Nonlesions, misdiagnoses, missed diagnoses, and other interpretive challenges in fish histopathology studies: A guide for investigators, authors, reviewers, and readers. Toxicologic Pathology, 43(3):297–325.
  48. Yilmaz, S. (2019). Effects of dietary caffeic acid supplement on antioxidant, immunological and liver gene expression responses, and resistance of Nile tilapia, Oreochromis niloticus to Aeromonas veronii. Fish & Shellfish Immunology, 86(11):384–392.
  49. Zahran, E., Risha, E., AbdelHamid, F., Mahgoub, H. A., & Ibrahim, T. (2014). Effects of dietary Astragalus polysaccharides (APS) on growth performance, immunological parameters, digestive enzymes, and intestinal morphology of Nile tilapia (Oreochromis niloticus). Fish & Shellfish Immunology, 38(1):149–157.
  50. Zhang, W., Zhao, J., Ma, Y., Li, J., & Chen, X. (2022). The effective components of herbal medicines used for prevention and control of fish diseases. Fish & Shellfish Immunology, 126:73–83.
  51. Zhang, X., Huang, K., Zhong, H., Ma, Y., Guo, Z., Tang, Z., Liang, J., Luo, Y., Su, Z., & Wang, L. (2020). Effects of Lycium barbarum polysaccharides on immunological parameters, apoptosis, and growth performance of Nile tilapia (Oreochromis niloticus). Fish & Shellfish Immunology, 97:509–514.
  52. Zhu, F. (2020). A review on the application of herbal medicines in the disease control of aquatic animals. Aquaculture, 526:735422.
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Author Biographies

Arini Resti Fauzi, Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Munti Yuhana, Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Widanarni Widanarni, Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Mia Setiawati, Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Usamah Afiff, Department of Veterinary Disease and Public Health, Veterinary and Biomedical School, IPB University, Bogor, West Java. Indonesia

Department of Veterinary Disease and Public Health, Veterinary and Biomedical School, IPB University, Bogor, West Java. Indonesia

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