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Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

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Fisheries Biotechnology

Growth and Metabolite Enhancement of Acidophile Euglena sp. Isolated from Indonesia under Different Photoperiod Cycles

https://doi.org/10.20473/jipk.v16i1.46193
Tia Erfianti
Faculty of Biology, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia
https://orcid.org/0000-0002-5462-1131
Budi Setiadi Daryono
Faculty of Biology, Universitas Gadjah MadaFaculty of Biology, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia
https://orcid.org/0000-0002-0703-2123
Arief Budiman
Department of Chemical Engineering, Faculty of Engineering, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia; Center of Excellent of Microalgae, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia
https://orcid.org/0000-0003-4846-8270
Eko Agus Suyono
Faculty of Biology, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia; Center of Excellent of Microalgae, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia
https://orcid.org/0000-0002-9208-4541

Corresponding Author(s) : Eko Agus Suyono

eko_suyono@ugm.ac.id

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract


Abstract


Euglena sp. is a unicellular, flagellated microalga considered one of the most promising microalgal feedstock species for biofuels. Reducing  the  level  of  liquid waste pollutants  can be  done  biologically  by  using  microalgal  organisms. Its metabolites, including lipids, proteins, carbohydrates, and pigments, are appropriate for producing biorefinery products such as biodiesel and jet fuels. They can be isolated from extreme environments, such as highly acidic and ammonia-rich environments, that are not conducive to their proliferation. This study sought to determine the effect of the photoperiod or (light: dark) cycle (24 L:0 D, 12 L:12 D, 14 L:10 D, and 16 L:8 D) on the growth, biomass, metabolite content consisting of lipids, carbohydrates, and proteins, and the rate of CO­2 uptake by Euglena sp. As stated previously, the study was conducted by cultivating Euglena sp. on a laboratory scale with four photoperiod regimens. The results indicated that optimal growth, biomass content, and metabolite content were obtained with a 24 D:0 L lighting cycle. The control treatment (24 L: 0 D) had the highest biomass productivity (0.032 g.L-1.day-1 ± 0.004), lipid content (0.387 g.L-1 ± 0.031), protein content (0.542 mg.Ml-1 ± 0.007), carbohydrate content (0.409 x104 g.L-1), chlorophyll a (6.237 g.L-1 ± 0.184), chlorophyll b (2.838 g.L-1 ± 0.253), and total carotenoid (1.566 g.L-1 ± 0.105). Full light illumination (24 L:0 D) was significantly producing carotenoid content, including phaeophytin a, phaeophytin b, violaxanthin, 9’-cis-neoxanthin, dino xanthin, and fucoxanthin.


Highlight Research



  1. The growth rate was higher when Euglena was cultivated under continuous illumination.

  2. The biomass productivity of Euglena increased significantly under continuous illumination.

  3. The metabolite content of Euglena (lipids, proteins, and pigments) was higher in continuous illumination.

Keywords

Carotenoids Euglena sp. growth rate metabolites photoperiod
Erfianti, T., Daryono, B. S., Budiman, A., & Suyono, E. A. (2023). Growth and Metabolite Enhancement of Acidophile Euglena sp. Isolated from Indonesia under Different Photoperiod Cycles. Jurnal Ilmiah Perikanan Dan Kelautan, 16(1), 15–30. https://doi.org/10.20473/jipk.v16i1.46193
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Author Biographies

Tia Erfianti, Faculty of Biology, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia

Faculty of Biology, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia

Budi Setiadi Daryono, Faculty of Biology, Universitas Gadjah MadaFaculty of Biology, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia

Faculty of Biology, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia

Arief Budiman, Department of Chemical Engineering, Faculty of Engineering, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia; Center of Excellent of Microalgae, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia

Department of Chemical Engineering, Faculty of Engineering, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia

Center of Excellent of Microalgae, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia

Eko Agus Suyono, Faculty of Biology, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia; Center of Excellent of Microalgae, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia

Faculty of Biology, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia

Center of Excellent of Microalgae, Universitas Gadjah Mada, Sleman, Yogyakarta, 55281. Indonesia

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