Abstract
Coomassie Brilliant Blue G-250 (CBBG) was bound to the reaction products of glutaraldehyde with various compounds containing amino group. The reaction products of acetaldehyde and malonaldehyde with the amines did not bind with CBBG, indicating that the binding was specific for the product of glutaraldehyde. The dye binding products rapidly formed in alkaline pH. Gel filtration chromatography indicated that a reaction product of more than 2 kDa has a dye-binding ability. The reaction product of glutaraldehyde with 6-aminohexanoic acid was separated with SP-Sephadex. CBBG was bound to the first eluted fraction from the column with highest affinity. The absorbance at 595 nm by CBBG binding was linearly related to this lyophilized fraction at a concentration of up to 250 μg/ml, suggesting its applicability to the determination of the reaction product of glutaraldehyde and amine. The present method should contribute to our understanding of the chemistry of glutaraldehyde associated with diverse fields, such as enzyme technology and biochemistry.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
H. Ukeda, M. Imabayashi, K. Matsumoto and Y. Osajima, Agric. Biol. Chem., 53, 2909 (1989).
T. J. A. Johnson, Eur. J. Cell Biol., 45, 160 (1987).
S. P. Gorman, E. M. Scott, and A. D. Russell, J. Appl. Bacterial., 48, 161 (1980).
H. Ukeda, E. Miyazaki, K. Matsumoto and Y. Osajima, Anal. Chem., 58, 2975 (1986).
H. Ukeda, K. Matsumoto, I. Katsuki and Y. Osajima, Nippon Nogeikagaku Kaishi, 65, 1229 (1991).
P. M. Hardy, A. C. Nicholls, and H. N. Rydon, J. Chem. Soc. Perkin Trans. 1, 1976, 958.
P. M. Hardy, G. J. Hughes, and H. N. Rydon, J. Chem. Soc. Perkin Trans. 1, 1979, 2282.
H. Ukeda, Y. Nakazono, K. Matsumoto and Y. Osajima, Biotechnol. Bioeng., 38, 948 (1991).
R. O. Beauchamp, Jr., M. B. G. St. Clair, T. R. Fennell, D. O. Clarke, and K. T. Morgan, Crit. Rev. Toxicol., 22, 143 (1992).
M. Imai, Y. Yanase, T. Nojiri, Y. Miyakawa and M. Mayumi, Gastroenterology, 76, 242 (1979).
A. Machida, S. Kishimoto, H. Ohnuma, H. Miyamoto, K. Baba, K. Oda, T. Nakamura, Y. Miyakawa and M. Mayumi, Gastroenterology, 85, 268 (1983).
M. Machida, H. Kishimoto, K. Ohnuma, K. Baba, Y. Ito, H. Miyamoto, G. Funatsu, K. Oda, S. Usuda, S. Togami, T. Nakamura, Y. Miyakawa and M. Mayumi, Gastroenterology, 86, 910 (1984).
T. Tsuji, I. Yamamoto, M. Tsuchiya and H. Nagashima, J. Immunol. Methods, 73, 357 (1984).
M. W. Yu, J. S. Finlayson, and J. W. K. Shih, J. Virol., 55, 736 (1985).
H. Ohnuma, K. Takahashi, S. Kishimoto, A. Machida, M. Imai, S. Mishiro, S. Usuda, K. Oda, T. Nakamura, Y. Miyakawa and M. Mayumi, Gastroenterology, 90, 695 (1986).
D. Onica, I. Margineanu, and R. Lenkei, Molec. Immun., 18, 807 (1981).
R. Lenkei, Rev. Roum. Med.-Med. Int., 18, 129 (1980).
M. M. Bradford, Anal. Biochem., 72, 248 (1976).
T. W. Kwon and B. M. Watts, J. FoodSci., 28, 627 (1963).
K. Satake, T. Okuyama, M. Ohashi and T. Shinoda, J. Biochem., 47, 654 (1960).
R. Fields, Biochem. J., 124, 581 (1971).
M. A. Paz, B. Pereyra, P. M. Gallop, S. Seifter, J. Mechanochem. Cell Motility, 2, 231 (1974).
S. J. Compton and C. G. Jones, Anal. Biochem., 151, 369 (1985).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ishii, T., Sorita, A., Sawamura, M. et al. Determination of the Reaction Product of Glutaraldehyde and Amine Based on the Binding Ability of Coomassie Brilliant Blue. ANAL. SCI. 13, 5–9 (1997). https://doi.org/10.2116/analsci.13.5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2116/analsci.13.5