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Estimation of the Antioxidant Activities of Flavonoids from Their Oxidation Potentials

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Abstract

A simple electrochemical method for estimating the antioxidant activity (AA) of flavonoids has been developed. The proposed method is based on a measurement of the half-wave potential (E1/2) of the first oxidation wave of flavonoids by using flow-through column electrolysis. At the same time, the lipid peroxidation (LPO) inhibiting effects of these flavonoids were determined. A quantitative structure–activity relationship was obtained to describe the AA of flavonoids: IC50(¼M) = 30.36 + 151.50E1/2 (V)–12.63log P (r = 0.852), where IC50 represents the concentration for 50% inhibition of LPO, and P represents the octanol/water partition coefficient. This method is expected to be useful for the quick screening of flavonoid antioxidants, and evaluating the AA of flavonoid-containing foods and medicinal plants.

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Authors and Affiliations

  1. Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing, 100700, China

    Bin Yang

  2. School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan

    Akira Kotani, Kensuke Arai & Fumiyo Kusu

Authors
  1. Bin Yang
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  2. Akira Kotani
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  3. Kensuke Arai
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  4. Fumiyo Kusu
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Correspondence to Fumiyo Kusu.

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Yang, B., Kotani, A., Arai, K. et al. Estimation of the Antioxidant Activities of Flavonoids from Their Oxidation Potentials. ANAL. SCI. 17, 599–604 (2001). https://doi.org/10.2116/analsci.17.599

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  • DOI: https://doi.org/10.2116/analsci.17.599

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